Rv3340 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3340 metC O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) CDS 3726127 3727476 + 1 350 449 FALSE

Rv3340 (O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)) is predicted to be co-regulated in modules bicluster_0023 with residual 0.49 and bicluster_0366 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 200.00 and 300.00 for bicluster_0023 and 0.00 and 0.00 for bicluster_0366 respectively.

These modules are enriched for following go terms: adenylyltransferase activity, transferase activity hydrolase activity, acting on ester bond....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE O-ACETYLHOMOSERINE SULFHYDRYLASE METC [HOMOCYSTEINE SYNTHASE] [O-ACETYLHOMOSERINE [THIOL]-LYASE] [OAH SULFHYDRYLASE] [O-ACETYL-L-HOMOSERINE SULFHYDRYLASE] O-acetylhomoserine aminocarboxypropyltransferase
Operon # Operon
2182 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Cysteine and methionine metabolism

28
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610476 NP_217857.1 Run
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.210000 0.26

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: