Rv3342 Methyltransferase (EC 2.1.1.-)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv3342 Methyltransferase (EC 2.1.1.-) CDS 3728624 3729355 + 732 243 FALSE

Rv3342 (Methyltransferase (EC 2.1.1.-)) is predicted to be co-regulated in modules bicluster_0366 with residual 0.53 and bicluster_0422 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0366 and 0.18 and 5,500.00 for bicluster_0422 respectively.

These modules are enriched for following go terms: hydrolase activity, acting on ester bond... .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS New Internal UTR Re-Annotated Start Tuberculist Annotated Start
-0.063 216 3728840 -195 3728645 3728624
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0366
e.value: 
0.000000032
Motif Bicluster: 
e.value: 
0.00074
Motif Bicluster: 
0.53
bicluster_0422
e.value: 
0.18
Motif Bicluster: 
e.value: 
5500
Motif Bicluster: 
0.52
Product (LegacyBRC) Product (RefSeq)
Uncharacterized methyltransferase Rv3342_MT3445 methyltransferase (methylase)
Operon # Operon
2182 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Histidine metabolism

40
Total items in this category:  

KEGG

Tyrosine metabolism

41
Total items in this category:  

KEGG

Selenocompound metabolism

40
Total items in this category:  

KEGG

Polycyclic aromatic hydrocarbon degradation

47
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610478 NP_217859.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: