Rv3373 Enoyl-CoA hydratase (EC 4.2.1.17)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3373 echA18 Enoyl-CoA hydratase (EC 4.2.1.17) CDS 3787726 3788367 + 642 213 FALSE

Rv3373 (Enoyl-CoA hydratase (EC 4.2.1.17)) is predicted to be co-regulated in modules bicluster_0437 with residual 0.55 and bicluster_0490 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 700.00 and 1,900.00 for bicluster_0437 and 0.01 and 730.00 for bicluster_0490 respectively.

These modules are enriched for following go terms: .

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-1.082 3787741 3787726
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0437
e.value: 
700
Motif Bicluster: 
e.value: 
1900
Motif Bicluster: 
0.55
bicluster_0490
e.value: 
0.0085
Motif Bicluster: 
e.value: 
730
Motif Bicluster: 
0.57
Product (LegacyBRC) Product (RefSeq)
PROBABLE ENOYL-CoA HYDRATASE ECHA18 [ENOYL HYDRASE] [UNSATURATED ACYL-CoA HYDRATASE] [CROTONASE] enoyl-CoA hydratase
Operon # Operon
2206 Rv3373 - Rv3374 - Rv3375
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid metabolism

51
Total items in this category:  

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

Geraniol degradation

52
Total items in this category:  

KEGG

Lysine degradation

46
Total items in this category:  

KEGG

Benzoate degradation

48
Total items in this category:  

KEGG

Tryptophan metabolism

47
Total items in this category:  

KEGG

beta-Alanine metabolism

37
Total items in this category:  

KEGG

Aminobenzoate degradation

73
Total items in this category:  

KEGG

Propanoate metabolism

62
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610509 NP_217890.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.380000 2.60

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.