Rv3375 Glutamyl-tRNA(Gln) amidotransferase subunit A-like protein

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3375 amiD Glutamyl-tRNA(Gln) amidotransferase subunit A-like protein CDS 3788621 3790048 + 1 428 475 FALSE

Rv3375 (Glutamyl-tRNA(Gln) amidotransferase subunit A-like protein) is predicted to be co-regulated in modules bicluster_0437 with residual 0.55 and bicluster_0490 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 700.00 and 1,900.00 for bicluster_0437 and 0.01 and 730.00 for bicluster_0490 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 15:27
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18100 MT3485 1406
Product (LegacyBRC) Product (RefSeq)
Putative amidase amiD amidase AmiD
Operon # Operon
2206 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Arginine and proline metabolism

38
Total items in this category:  

KEGG

Phenylalanine metabolism

13
Total items in this category:  

KEGG

Tryptophan metabolism

47
Total items in this category:  

KEGG

Cyanoamino acid metabolism

12
Total items in this category:  

KEGG

Aminobenzoate degradation

73
Total items in this category:  

KEGG

Styrene degradation

5
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610511 NP_217892.1 Run
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.390000 1.28

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: