Rv3406 Alpha-ketoglutarate-dependent taurine dioxygenase (EC 1.14.11.17)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv3406 Alpha-ketoglutarate-dependent taurine dioxygenase (EC 1.14.11.17) CDS 3825330 3826217 + 888 295 FALSE

Rv3406 (Alpha-ketoglutarate-dependent taurine dioxygenase (EC 1.14.11.17)) is predicted to be co-regulated in modules bicluster_0043 with residual 0.47 and bicluster_0580 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.01 for bicluster_0043 and 0.00 and 0.19 for bicluster_0580 respectively.

These modules are enriched for following go terms: nucleic acid binding transcription facto..., sequence-specific DNA binding transcript... .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 15:33
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18312 MT3514 1990
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, ArsR family
Induced -33 1.48 0.0000000000433 CDS
Transcriptional regulator, TetR family
No -36 -1.17 0.0555903 CDS
Motif 1 Motif 2 Residual
bicluster_0043
e.value: 
0.00000015
Motif Bicluster: 
e.value: 
0.0062
Motif Bicluster: 
0.47
bicluster_0580
e.value: 
0.0000012
Motif Bicluster: 
e.value: 
0.19
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
Uncharacterized dioxygenase Rv3406_MT3514 dioxygenase
Operon # Operon
2229 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Taurine dioxygenase Taurine and hypotaurine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Taurine and hypotaurine metabolism

7
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610542 NP_217923.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0004065

arylsulfatase activity

arylsulfatase activity

Details: 
Catalysis of the reaction: a phenol sulfate + H2O = a phenol + sulfate.
GO Category: 
molecular_function
3
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.540000 1.02

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: