Rv3407

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3407 vapB47 CDS 3826252 3826551 + 300 99 FALSE

Rv3407 () is predicted to be co-regulated in modules bicluster_0087 with residual 0.55 and bicluster_0276 with residual 0.47.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 590.00 and 1,300.00 for bicluster_0087 and 93.00 and 170.00 for bicluster_0276 respectively.

These modules are enriched for following go terms: drug transmembrane transporter activity, drug transporter activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv3407_MT3515
Operon # Operon
2229 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics
Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610543 NP_217924.1 Run
GO:0045927

positive regulation of growth

positive regulation of growth

Details: 
Any process that activates or increases the rate or extent of growth, the increase in size or mass of all or part of an organism.
GO Category: 
biological_process
31
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.260000 0.45

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: