Rv3420c Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3420c rimI Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-) CDS 3838586 3839062 - 477 158 FALSE

Rv3420c (Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-)) is predicted to be co-regulated in modules bicluster_0192 with residual 0.54 and bicluster_0460 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 4,500.00 and 12,000.00 for bicluster_0192 and 55.00 and 160.00 for bicluster_0460 respectively.

These modules are enriched for following go terms: cellular macromolecular complex assembly, cellular protein complex assembly, protein complex assembly, macromolecular complex assembly, protein complex biogenesis branched-chain amino acid metabolic proc..., translation, acetolactate synthase activity, anion transmembrane-transporting ATPase ..., structural constituent of ribosome.

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No -70 -0.09 0.999609 Internal.TSS
Motif 1 Motif 2 Residual
bicluster_0192
e.value: 
4500
Motif Bicluster: 
e.value: 
12000
Motif Bicluster: 
0.54
bicluster_0460
e.value: 
55
Motif Bicluster: 
e.value: 
160
Motif Bicluster: 
0.59
Product (LegacyBRC) Product (RefSeq)
PROBABLE RIBOSOMAL-PROTEIN-ALANINE ACETYLTRANSFERASE RIMI [ACETYLATING ENZYME FOR N-TERMINAL OF RIBOSOMAL PROTEIN S18] ribosomal-protein-alanine acetyltransferase
Operon # Operon
2237 - - - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610556 NP_217937.1 Run
GO:0008080

N-acetyltransferase activity

N-acetyltransferase activity

Details: 
Catalysis of the transfer of an acetyl group to a nitrogen atom on the acceptor molecule.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.030000 0.60

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: