Rv3464 dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3464 rmlB dTDP-glucose 4,6-dehydratase (EC 4.2.1.46) CDS 3881837 3882832 + 996 331 FALSE

Rv3464 (dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)) is predicted to be co-regulated in modules bicluster_0255 with residual 0.52 and bicluster_0491 with residual 0.60.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 3.80 for bicluster_0255 and 0.01 and 4,200.00 for bicluster_0491 respectively.

These modules are enriched for following go terms: .

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No -65 -0.06 0.818237 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0255
e.value: 
0.000011
Motif Bicluster: 
e.value: 
3.8
Motif Bicluster: 
0.52
bicluster_0491
e.value: 
0.0056
Motif Bicluster: 
e.value: 
4200
Motif Bicluster: 
0.60
Product (LegacyBRC) Product (RefSeq)
dTDP-glucose 46-dehydratase dTDP-glucose 4,6-dehydratase RMLB
Operon # Operon
2264 Rv3464 - Rv3465
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Streptomycin biosynthesis

12
Total items in this category:  

KEGG

Polyketide sugar unit biosynthesis

6
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610600 NP_217981.1 Run
GO:0008460

dTDP-glucose 4,6-dehydratase activity

dTDP-glucose 4,6-dehydratase activity

Details: 
Catalysis of the reaction: dTDP-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H(2)O.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.