Rv3465 dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3465 rmlC dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13) CDS 3882834 3883442 + 609 202 FALSE

Rv3465 (dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13)) is predicted to be co-regulated in modules bicluster_0255 with residual 0.52 and bicluster_0491 with residual 0.60.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 3.80 for bicluster_0255 and 0.01 and 4,200.00 for bicluster_0491 respectively.

These modules are enriched for following go terms: .

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0255
e.value: 
0.000011
Motif Bicluster: 
e.value: 
3.8
Motif Bicluster: 
0.52
bicluster_0491
e.value: 
0.0056
Motif Bicluster: 
e.value: 
4200
Motif Bicluster: 
0.60
Product (LegacyBRC) Product (RefSeq)
dTDP-4-DEHYDRORHAMNOSE 35-EPIMERASE RMLC [DTDP-4-KETO-6-DEOXYGLUCOSE 35-EPIMERASE] [DTDP-L-RHAMNOSE SYNTHETASE] [THYMIDINE DIPHOSPHO-4-KETO-RHAMNOSE 35-EPIMERASE] dTDP-4-dehydrorhamnose 3,5-epimerase RmlC
Operon # Operon
2264 Rv3464 - Rv3465
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Streptomycin biosynthesis

12
Total items in this category:  

KEGG

Polyketide sugar unit biosynthesis

6
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610601 NP_217982.1 Run
GO:0008830

dTDP-4-dehydrorhamnose 3,5-epimerase activity

dTDP-4-dehydrorhamnose 3,5-epimerase activity

Details: 
Catalysis of the reaction: dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-L-mannose.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0008830

dTDP-4-dehydrorhamnose 3,5-epimerase activity

dTDP-4-dehydrorhamnose 3,5-epimerase activity

Details: 
Catalysis of the reaction: dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-L-mannose.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0019305

dTDP-rhamnose biosynthetic process

dTDP-rhamnose biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of dTDP-rhamnose, a substance composed of rhamnose in glycosidic linkage with deoxyribosylthymine diphosphate.
GO Category: 
biological_process
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.