Rv3487c putative esterase

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3487c lipF putative esterase CDS 3906174 3907007 - 834 277 FALSE

Rv3487c (putative esterase) is predicted to be co-regulated in modules bicluster_0325 with residual 0.54 and bicluster_0349 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 72.00 for bicluster_0325 and 2.40 and 4,600.00 for bicluster_0349 respectively.

These modules are enriched for following go terms: gene expression, macromolecule metabolic process, cellular macromolecule biosynthetic proc..., macromolecule biosynthetic process, ribosome, ribonucleoprotein complex, non-membrane-bounded organelle, intracellular non-membrane-bounded organ..., nucleotidyltransferase activity lipopolysaccharide metabolic process, lipopolysaccharide biosynthetic process, carbon-carbon lyase activity, oxo-acid-lyase activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Distance to Tuberculist Start Codon Internal TSS Re-Annotated Start Tuberculist Annotated Start
-0.202 530 3906477 3907031 3907007
Last update: 10/16/2017 - 15:33
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15151 MT3591 2029
Product (LegacyBRC) Product (RefSeq)
PROBABLE ESTERASE_LIPASE LIPF esterase/lipase LipF
Operon # Operon
2281
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610623 NP_218004.1 Run
GO:0004091

carboxylesterase activity

carboxylesterase activity

Details: 
Catalysis of the reaction: a carboxylic ester + H2O = an alcohol + a carboxylic anion.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0004629

phospholipase C activity

phospholipase C activity

Details: 
Catalysis of the reaction: a phospholipid + H2O = 1,2-diacylglycerol + a phosphatidate.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0010447

response to acidity

response to acidity

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a pH stimulus with pH < 7.
GO Category: 
biological_process
2
Total items in this category:  
GO:0044119

growth of symbiont in host cell

growth of symbiont in host cell

Details: 
The increase in size or mass of symbiont, occurring in its host's cell. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
50
Total items in this category:  
GO:0052572

response to host immune response

response to host immune response

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of detecting the immune response of the host organism. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
62
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.800000 1.13

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: