Rv3535c Acetaldehyde dehydrogenase, acetylating, (EC 1.2.1.10) in gene cluster for degradation of phenols, cresols, catechol

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3535c hsaG Acetaldehyde dehydrogenase, acetylating, (EC 1.2.1.10) in gene cluster for degradation of phenols,... CDS 3973589 3974500 - 912 303 FALSE

Rv3535c (Acetaldehyde dehydrogenase, acetylating, (EC 1.2.1.10) in gene cluster for degradation of phenols, cresols, catechol) is predicted to be co-regulated in modules bicluster_0199 with residual 0.54 and bicluster_0337 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0199 and 0.00 and 0.00 for bicluster_0337 respectively.

These modules are enriched for following go terms: aspartate carbamoyltransferase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE ACETALDEHYDE DEHYDROGENASE [ACETALDEHYDE DEHYDROGENASE [ACETYLATING]] acetaldehyde dehydrogenase
Operon # Operon
2309 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Benzoate degradation

48
Total items in this category:  

KEGG

Pyruvate metabolism

40
Total items in this category:  

KEGG

Dioxin degradation

5
Total items in this category:  

KEGG

Xylene degradation

4
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610671 NP_218052.1 Run
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.020000 0.32

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: