Rv3588c Carbonic anhydrase (EC 4.2.1.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3588c canB Carbonic anhydrase (EC 4.2.1.1) CDS 4029871 4030494 - 624 207 FALSE

Rv3588c (Carbonic anhydrase (EC 4.2.1.1)) is predicted to be co-regulated in modules bicluster_0154 with residual 0.56 and bicluster_0349 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 6.50 and 19,000.00 for bicluster_0154 and 2.40 and 4,600.00 for bicluster_0349 respectively.

These modules are enriched for following go terms: nitrate reductase complex, nitrate reductase activity, oxidoreductase activity, acting on other... lipopolysaccharide metabolic process, lipopolysaccharide biosynthetic process, carbon-carbon lyase activity, oxo-acid-lyase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Carbonic anhydrase carbonic anhydrase
Operon # Operon
2337
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Carbonate dehydratase Nitrogen metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Nitrogen metabolism

24
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610724 NP_218105.1 Run
GO:0004089

carbonate dehydratase activity

carbonate dehydratase activity

Details: 
Catalysis of the reaction: H2CO3 = CO2 + H2O.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0004089

carbonate dehydratase activity

carbonate dehydratase activity

Details: 
Catalysis of the reaction: H2CO3 = CO2 + H2O.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0008270

zinc ion binding

zinc ion binding

Details: 
Interacting selectively and non-covalently with zinc (Zn) ions.
GO Category: 
molecular_function
15
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
GO:0051289

protein homotetramerization

protein homotetramerization

Details: 
The formation of a protein homotetramer, a macromolecular structure consisting of four noncovalently associated identical subunits.
GO Category: 
biological_process
17
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: