Rv3624c Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3624c hpt Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) CDS 4063254 4063904 - 651 216 FALSE

Rv3624c (Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8)) is predicted to be co-regulated in modules bicluster_0150 with residual 0.60 and bicluster_0481 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.46 and 72.00 for bicluster_0150 and 0.04 and 0.31 for bicluster_0481 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Distance to Tuberculist Start Codon Internal TSS New Internal UTR Re-Annotated Start Tuberculist Annotated Start
-0.777 48 4063856 -6 4063862 4063904
Last update: 10/16/2017 - 16:15
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14958 MT3726 360
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 6 of 6
ChipSeq TF Differential Expression Distance Expression pvalue Type
Organic hydroperoxide resistance transcriptional regulator
No -41 0.13 0.670631 CDS
Organic hydroperoxide resistance transcriptional regulator
No 7 0.13 0.670631 Internal.TSS
Transcriptional regulator, PadR family
No -20 0.1 0.766175 Antisense.TSS
Transcriptional regulator, TetR family
No 40 0 0.99568 Antisense.TSS
Transcriptional regulator, TetR family
No -14 0.24 0.40151 Antisense.TSS
No 22 0.22 0.522808 Antisense.TSS
Motif 1 Motif 2 Residual
bicluster_0150
e.value: 
0.46
Motif Bicluster: 
e.value: 
72
Motif Bicluster: 
0.60
bicluster_0481
e.value: 
0.036
Motif Bicluster: 
e.value: 
0.31
Motif Bicluster: 
0.57
Product (LegacyBRC) Product (RefSeq)
Hypoxanthine-guanine phosphoribosyltransferase hypoxanthine-guanine phosphoribosyltransferase
Operon # Operon
2358 - - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610760 NP_218141.1 Run
GO:0004422

hypoxanthine phosphoribosyltransferase activity

hypoxanthine phosphoribosyltransferase activity

Details: 
Catalysis of the reaction: IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GO Category: 
molecular_function
1
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: