Rv3661 Phosphoserine phosphatase (EC 3.1.3.3)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv3661 Phosphoserine phosphatase (EC 3.1.3.3) CDS 4099647 4100510 + 864 287 FALSE

Rv3661 (Phosphoserine phosphatase (EC 3.1.3.3)) is predicted to be co-regulated in modules bicluster_0171 with residual 0.49 and bicluster_0379 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.77 and 120.00 for bicluster_0171 and 8.60 and 9.10 for bicluster_0379 respectively.

These modules are enriched for following go terms: NAD(P)+ transhydrogenase activity, oxidoreductase activity, acting on NAD(P....

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 16:15
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14914 MT3761 235
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 10 of 13
ChipSeq TF Differential Expression Distance Expression pvalue Type
transcriptional regulator, ArsR family
No -113 0.37 0.00000615 Primary.TSS
Transcriptional regulator, TetR family
No 63 0.09 0.833612 Primary.TSS
DNA-binding response regulator TrcR
No -110 0.3 0.0714011 Primary.TSS
Transcriptional regulator, ArsR family
Repressed -132 -0.76 0.0000000000877 Primary.TSS
Transcriptional regulator, TetR family
No -137 0.15 0.426339 Primary.TSS
Transcriptional regulator, AraC family
No -100 0.29 0.321172 Primary.TSS
Transcriptional regulator, AsnC family
No -119 -0.14 0.820277 Primary.TSS
Transcriptional regulator, MarR family
No -124 -0.41 0.0012595 Primary.TSS
Transcriptional regulator, TetR family
No -99 0.15 0.377597 Primary.TSS
transcriptional regulator, ArsR family
No -147 0 0.999838 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0171
e.value: 
0.77
Motif Bicluster: 
e.value: 
120
Motif Bicluster: 
0.49
bicluster_0379
e.value: 
8.6
Motif Bicluster: 
e.value: 
9.1
Motif Bicluster: 
0.53
Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv3661_MT3761
Operon # Operon
2383
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610797 NP_218178.1 Run
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.880000 1.46

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: