Rv3677c FIG146518: Zn-dependent hydrolases, including glyoxylases

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv3677c FIG146518: Zn-dependent hydrolases, including glyoxylases CDS 4117258 4118052 - 795 264 FALSE

Rv3677c (FIG146518: Zn-dependent hydrolases, including glyoxylases) is predicted to be co-regulated in modules bicluster_0030 with residual 0.60 and bicluster_0382 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.05 and 0.81 for bicluster_0030 and 0.00 and 0.26 for bicluster_0382 respectively.

These modules are enriched for following go terms: single-organism metabolic process, single-organism cellular process, biosynthetic process, cellular nitrogen compound metabolic pro..., nitrogen compound metabolic process, membrane part .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 16:15
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18667 MT3778 2802
Product (LegacyBRC) Product (RefSeq)
POSSIBLE HYDROLASE hydrolase
Operon # Operon
2393 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610813 NP_218194.1 Run
GO:0008800

beta-lactamase activity

beta-lactamase activity

Details: 
Catalysis of the reaction: a beta-lactam + H2O = a substituted beta-amino acid.
GO Category: 
molecular_function
5
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.290000 0.70

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: