Rv3757c L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3757c proW L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1) CDS 4202610 4203299 - 690 229 FALSE

Rv3757c (L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1)) is predicted to be co-regulated in modules bicluster_0176 with residual 0.50 and bicluster_0433 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.08 and 1.60 for bicluster_0176 and 0.00 and 0.00 for bicluster_0433 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
POSSIBLE OSMOPROTECTANT [GLYCINE BETAINE_CARNITINE_CHOLINE_L-PROLINE] TRANSPORT INTEGRAL MEMBRANE PROTEIN ABC TRANSPORTER PROW osmoprotectant (glycine betaine/carnitine/choline/L-proline) transport integral membrane protein ABC transporter PROW
Operon # Operon
2455 - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

ABC transporters

57
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610893 NP_218274.1 Run
GO:0005215

transporter activity

transporter activity

Details: 
Enables the directed movement of substances (such as macromolecules, small molecules, ions) into, out of or within a cell, or between cells.
GO Category: 
molecular_function
27
Total items in this category:  
GO:0006810

transport

transport

Details: 
The directed movement of substances (such as macromolecules, small molecules, ions) into, out of or within a cell, or between cells, or within a multicellular organism by means of some agent such as a transporter or pore.
GO Category: 
biological_process
28
Total items in this category:  
GO:0016020

membrane

membrane

Details: 
Double layer of lipid molecules that encloses all cells, and, in eukaryotes, many organelles; may be a single or double lipid bilayer; also includes associated proteins.
GO Category: 
cellular_component
33
Total items in this category:  
GO:0005488

binding

binding

Details: 
The selective, non-covalent, often stoichiometric, interaction of a molecule with one or more specific sites on another molecule.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0031460

glycine betaine transport

glycine betaine transport

Details: 
The directed movement of glycine betaine, N-trimethylglycine, into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
GO Category: 
biological_process
4
Total items in this category:  
GO:0075136

response to host

response to host

Details: 
Any process that results in a change in state or activity of the symbiont or its cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of detecting molecules of its host organism. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
12
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.200000 1.05

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: