Rv3759c L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3759c proX L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1) CDS 4204426 4205373 - 948 315 FALSE

Rv3759c (L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1)) is predicted to be co-regulated in modules bicluster_0413 with residual 0.50 and bicluster_0433 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.12 and 0.15 for bicluster_0413 and 0.00 and 0.00 for bicluster_0433 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
POSSIBLE OSMOPROTECTANT [GLYCINE BETAINE_CARNITINE_CHOLINE_L-PROLINE] BINDING LIPOPROTEIN PROX osmoprotectant (glycine betaine/carnitine/choline/L-proline) binding lipoprotein PROX
Operon # Operon
2455 - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

ABC transporters

57
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610895 NP_218276.1 Run
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0031460

glycine betaine transport

glycine betaine transport

Details: 
The directed movement of glycine betaine, N-trimethylglycine, into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
GO Category: 
biological_process
4
Total items in this category:  
GO:0075136

response to host

response to host

Details: 
Any process that results in a change in state or activity of the symbiont or its cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of detecting molecules of its host organism. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
12
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.460000 0.61

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: