Rv3772 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3772 hisC2 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57) CDS 4217134 4218195 + 1 062 353 FALSE

Rv3772 (Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57)) is predicted to be co-regulated in modules bicluster_0123 with residual 0.55 and bicluster_0141 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2.70 and 4,200.00 for bicluster_0123 and 0.31 and 1,600.00 for bicluster_0141 respectively.

These modules are enriched for following go terms: small molecule biosynthetic process, single-organism biosynthetic process, fatty acid biosynthetic process, carboxylic acid metabolic process, fatty acid synthase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0123
e.value: 
2.7
Motif Bicluster: 
e.value: 
4200
Motif Bicluster: 
0.55
bicluster_0141
e.value: 
0.31
Motif Bicluster: 
e.value: 
1600
Motif Bicluster: 
0.53
Product (LegacyBRC) Product (RefSeq)
Putative phenylalanine aminotransferase putative aminotransferase
Operon # Operon
2470
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Histidine metabolism

40
Total items in this category:  

KEGG

Tyrosine metabolism

41
Total items in this category:  

KEGG

Phenylalanine metabolism

13
Total items in this category:  

KEGG

Phenylalanine, tyrosine and tryptophan biosynthesis

18
Total items in this category:  

KEGG

Novobiocin biosynthesis

3
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610908 NP_218289.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.920000 1.84

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: