Rv3808c Galactofuranosyl transferase (EC 2.-.-.-)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3808c glfT2 Galactofuranosyl transferase (EC 2.-.-.-) CDS 4270366 4272279 - 1 914 637 FALSE

Rv3808c (Galactofuranosyl transferase (EC 2.-.-.-)) is predicted to be co-regulated in modules bicluster_0034 with residual 0.54 and bicluster_0384 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 57.00 and 260.00 for bicluster_0034 and 0.00 and 0.58 for bicluster_0384 respectively.

These modules are enriched for following go terms: phosphotransferase activity, for other s... .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0034
e.value: 
57
Motif Bicluster: 
e.value: 
260
Motif Bicluster: 
0.54
bicluster_0384
e.value: 
0.0002
Motif Bicluster: 
e.value: 
0.58
Motif Bicluster: 
0.49
Product (LegacyBRC) Product (RefSeq)
BIFUNCTIONAL UDP-GALACTOFURANOSYL TRANSFERASE GLFT bifunctional UDP-galactofuranosyl transferase GLFT
Operon # Operon
2491 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Transferases. Puromycin biosynthesis, Lipopolysaccharide biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610944 NP_218325.1 Run
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0008921

lipopolysaccharide-1,6-galactosyltransferase activity

lipopolysaccharide-1,6-galactosyltransferase activity

Details: 
Catalysis of the reaction: UDP-galactose + lipopolysaccharide = UDP + 1,6 alpha-D-galactosyl-lipopolysaccharide.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0035250

UDP-galactosyltransferase activity

UDP-galactosyltransferase activity

Details: 
Catalysis of the transfer of a galactose group from UDP-galactose to an acceptor molecule.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0035496

lipopolysaccharide-1,5-galactosyltransferase activity

lipopolysaccharide-1,5-galactosyltransferase activity

Details: 
Catalysis of the reaction: UDP-galactose + lipopolysaccharide = UDP + 1,5 alpha-D-galactosyl-lipopolysaccharide.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0070592

cell wall polysaccharide biosynthetic process

cell wall polysaccharide biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of a polysaccharide destined to form part of a cell wall.
GO Category: 
biological_process
1
Total items in this category:  
GO:0071769

mycolate cell wall layer assembly

mycolate cell wall layer assembly

Details: 
The aggregation, arrangement and bonding together of a set of components, including arabinogalactan mycolate and trehalose dimycolate, to form the mycolate layer of the Actinobacterium-type cell wall. The mycolate layer is physically attached to the peptidoglycan layer.
GO Category: 
biological_process
6
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: