Rv3809c UDP-galactopyranose mutase (EC 5.4.99.9)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3809c glf UDP-galactopyranose mutase (EC 5.4.99.9) CDS 4272276 4273475 - 1 200 399 FALSE

Rv3809c (UDP-galactopyranose mutase (EC 5.4.99.9)) is predicted to be co-regulated in modules bicluster_0292 with residual 0.50 and bicluster_0384 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.03 for bicluster_0292 and 0.00 and 0.58 for bicluster_0384 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.314 4 4273470 4273466 4273475
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, AraC family
No 38 0.03 0.987063 Primary.TSS
Transcriptional regulator kstR (Rv3574), TetR family
No 66 -0.1 0.714869 Primary.TSS
Beta-carotene ketolase (EC 1.14.-.-)
No 6 0.06 0.999473 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0292
e.value: 
0.000031
Motif Bicluster: 
e.value: 
0.031
Motif Bicluster: 
0.50
bicluster_0384
e.value: 
0.0002
Motif Bicluster: 
e.value: 
0.58
Motif Bicluster: 
0.49
Product (LegacyBRC) Product (RefSeq)
UDP-GALACTOPYRANOSE MUTASE GLF [UDP-GALP MUTASE] [NAD+-FLAVIN ADENINE DINUCLEOTIDE-REQUIRING ENZYME] UDP-galactopyranose mutase Glf
Operon # Operon
2491 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610945 NP_218326.1 Run
GO:0008767

UDP-galactopyranose mutase activity

UDP-galactopyranose mutase activity

Details: 
Catalysis of the reaction: UDP-D-galactopyranose = UDP-D-galacto-1,4-furanose.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0008767

UDP-galactopyranose mutase activity

UDP-galactopyranose mutase activity

Details: 
Catalysis of the reaction: UDP-D-galactopyranose = UDP-D-galacto-1,4-furanose.
GO Category: 
molecular_function
1
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: