Rv3874 10 kDa culture filtrate antigen CFP-10 (EsxB)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3874 esxB 10 kDa culture filtrate antigen CFP-10 (EsxB) CDS 4352274 4352576 + 303 100 FALSE

Rv3874 (10 kDa culture filtrate antigen CFP-10 (EsxB)) is predicted to be co-regulated in modules bicluster_0243 with residual 0.50 and bicluster_0559 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.06 and 2,000.00 for bicluster_0243 and 0.00 and 0.00 for bicluster_0559 respectively.

These modules are enriched for following go terms: Mo-molybdopterin cofactor biosynthetic p..., Mo-molybdopterin cofactor metabolic proc..., molybdopterin cofactor biosynthetic proc..., molybdopterin cofactor metabolic process, prosthetic group metabolic process.

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, MarR family
No -40 0.09 0.909583 Primary.TSS
No -9 0.16 0.794148 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0243
e.value: 
0.062
Motif Bicluster: 
e.value: 
2000
Motif Bicluster: 
0.50
bicluster_0559
e.value: 
0.0000032
Motif Bicluster: 
e.value: 
0.0049
Motif Bicluster: 
0.55
Product (LegacyBRC) Product (RefSeq)
ESAT-6-like protein esxB 10 kDa culture filtrate antigen EsxB
Operon # Operon
2533 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15611010 NP_218391.1 Run
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0009405

pathogenesis

pathogenesis

Details: 
The set of specific processes that generate the ability of an organism to cause disease in another.
GO Category: 
biological_process
65
Total items in this category:  
GO:0046812

host cell surface binding

host cell surface binding

Details: 
Interacting selectively and non-covalently with the surface of a host cell.
GO Category: 
molecular_function
6
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.200000 1.15

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: