Rv3875 6 kDa early secretory antigenic target ESAT-6 (EsxA)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3875 esxA 6 kDa early secretory antigenic target ESAT-6 (EsxA) CDS 4352609 4352896 + 288 95 FALSE

Rv3875 (6 kDa early secretory antigenic target ESAT-6 (EsxA)) is predicted to be co-regulated in modules bicluster_0472 with residual 0.59 and bicluster_0559 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 1.70 for bicluster_0472 and 0.00 and 0.00 for bicluster_0559 respectively.

These modules are enriched for following go terms: Mo-molybdopterin cofactor biosynthetic p..., Mo-molybdopterin cofactor metabolic proc..., molybdopterin cofactor biosynthetic proc..., molybdopterin cofactor metabolic process, prosthetic group metabolic process.

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
Histone protein Lsr2
No 40 0.12 0.896857 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0472
e.value: 
0.0071
Motif Bicluster: 
e.value: 
1.7
Motif Bicluster: 
0.59
bicluster_0559
e.value: 
0.0000032
Motif Bicluster: 
e.value: 
0.0049
Motif Bicluster: 
0.55
Product (LegacyBRC) Product (RefSeq)
6 kDa early secretory antigenic target 6 kDa early secretory antigenic target ESXA (ESAT-6)
Operon # Operon
2533 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117165 YP_178023.1 Run
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005737

cytoplasm

cytoplasm

Details: 
All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
GO Category: 
cellular_component
7
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0009405

pathogenesis

pathogenesis

Details: 
The set of specific processes that generate the ability of an organism to cause disease in another.
GO Category: 
biological_process
65
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
GO:0046812

host cell surface binding

host cell surface binding

Details: 
Interacting selectively and non-covalently with the surface of a host cell.
GO Category: 
molecular_function
6
Total items in this category:  
GO:0052027

modulation by symbiont of host signal transduction pathway

modulation by symbiont of host signal transduction pathway

Details: 
Any process in which an organism modulates the frequency, rate or extent of the host signal transduction pathways, the cascade of processes by which a signal interacts with a receptor. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
3
Total items in this category:  
GO:0052083

negative regulation by symbiont of host cell-mediated immune response

negative regulation by symbiont of host cell-mediated immune response

Details: 
Any process in which an organism stops, prevents, or reduces the frequency, rate or extent of the cell mediated immune response of the host organism. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
5
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: