Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_3226

Silent information regulator protein, Sir2 (NCBI)

CircVis
Functional Annotations (6)
Function System
NAD-dependent protein deacetylases, SIR2 family cog/ cog
DNA binding go/ molecular_function
chromatin silencing complex go/ cellular_component
chromatin silencing go/ biological_process
Amino sugar and nucleotide sugar metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_3226
(Mouseover regulator name to see its description)

RSP_3226 is regulated by 21 influences and regulates 8 modules.
Regulators for RSP_3226 (21)
Regulator Module Operator
RSP_0087 381 tf
RSP_0090 381 tf
RSP_0402 381 tf
RSP_0415 381 tf
RSP_1034 381 tf
RSP_1243 381 tf
RSP_1297 381 tf
RSP_1550 381 tf
RSP_1590 381 tf
RSP_1663 381 tf
RSP_2171 381 tf
RSP_2591 381 tf
RSP_2838 381 tf
RSP_2840 381 tf
RSP_2882 381 tf
RSP_2922 381 tf
RSP_3202 381 tf
RSP_3226 381 tf
RSP_0090 99 tf
RSP_1014 99 tf
RSP_3226 99 tf

Warning: RSP_3226 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7918 4.10e+03 AaTAAA
Loader icon
7919 1.60e+03 AttCgtT
Loader icon
8452 3.00e-01 gtCgGGccGCgGcggCggC.at
Loader icon
8453 3.30e+00 CatcGGaggcTCCttctTcC
Loader icon
Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_3226

RSP_3226 is enriched for 6 functions in 3 categories.
Enrichment Table (6)
Function System
NAD-dependent protein deacetylases, SIR2 family cog/ cog
DNA binding go/ molecular_function
chromatin silencing complex go/ cellular_component
chromatin silencing go/ biological_process
Amino sugar and nucleotide sugar metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Module neighborhood information for RSP_3226

RSP_3226 has total of 40 gene neighbors in modules 99, 381
Gene neighbors (40)
Gene Common Name Description Module membership
RSP_0090 smoC Operon regulator SmoC (NCBI) 99, 358
RSP_0282 ppsR Transcriptional regulator, PpsR (NCBI) 358, 381
RSP_0384 RSP_0384 hypothetical protein (NCBI) 329, 381
RSP_0415 RSP_0415 sigma-24 (NCBI) 308, 381
RSP_0592 RSP_0592 Putative lipase/esterase (NCBI) 99, 213
RSP_0599 RSP_0599 Probable DNA/pantothenate metabolism flavoprotein (NCBI) 242, 381
RSP_0708 RSP_0708 NUDIX hydrolase (NCBI) 242, 381
RSP_0710 dnaX DNA polymerase III tau and gamma subunits (NCBI) 173, 381
RSP_1188 wcaG Nucleotide sugar epimerase/dehydratase (NCBI) 59, 381
RSP_1211 RSP_1211 None 99, 308
RSP_1214 RSP_1214 putative branched-chain amino acid aminotransferase (NCBI) 215, 381
RSP_1215 ilvE putative IlvE, Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase (NCBI) 68, 381
RSP_1342 dnaA chromosomal replication initiator protein, DnaA (NCBI) 25, 381
RSP_1404 RSP_1404 Orotidine 5'-phosphate decarboxylase (NCBI) 311, 381
RSP_1405 RSP_1405 ROK family protein (NCBI) 285, 381
RSP_1520 prrB Sensor histidine kinase PrrB (RegB) (NCBI) 16, 381
RSP_1553 RSP_1553 Probable glyoxylate induced protein (NCBI) 102, 381
RSP_1554 RSP_1554 Possible polysaccharide deacetylase (NCBI) 102, 381
RSP_1555 RSP_1555 Transthyretin-like protein (NCBI) 329, 381
RSP_1566 RSP_1566 hypothetical protein (NCBI) 176, 381
RSP_1621 RSP_1621 Deoxyguanosinetriphosphate triphosphohydrolase-like protein (NCBI) 93, 381
RSP_1894 RSP_1894 hypothetical protein (NCBI) 323, 381
RSP_1908 RSP_1908 outer membrane protein, OmpA/MotB family (NCBI) 72, 381
RSP_1909 RSP_1909 Outer membrane general secretion pathway protein, Secretin (NCBI) 221, 381
RSP_1920 RSP_1920 Pyridoxal/pyridoxine/pyridoxamine kinase (NCBI) 99, 253
RSP_1921 RSP_1921 MOSC domain protein (NCBI) 99, 253
RSP_1946 RSP_1946 Cytochrome P450 hydroxylase (NCBI) 175, 381
RSP_2119 RSP_2119 hypothetical protein (NCBI) 10, 381
RSP_2694 RSP_2694 hypothetical protein (NCBI) 357, 381
RSP_2837 ntrB Nitrogen regulation protein, NtrB signal transduction histidine kinase (NCBI) 99, 318
RSP_2839 ntrY Nitrogen regulation protein, NtrY, Signal transduction histidine kinase (NCBI) 99, 318
RSP_2869 RSP_2869 Possible tellurite resistance protein (NCBI) 329, 381
RSP_2882 RSP_2882 two component transcriptional regulator, LuxR family (NCBI) 62, 381
RSP_2942 RSP_2942 hypothetical protein (NCBI) 349, 381
RSP_3010 RSP_3010 None 99, 178
RSP_3226 RSP_3226 Silent information regulator protein, Sir2 (NCBI) 99, 381
RSP_3376 aroQ 3 dehydroquinase dehydratase, class II (NCBI) 240, 381
RSP_3419 RSP_3419 hypothetical protein (NCBI) 175, 381
RSP_3507 RSP_3507 hypothetical protein (NCBI) 381, 384
RSP_3553 dnaE DNA polymerase III alpha chain (NCBI) 291, 381
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_3226
Please add your comments for this gene by using the form below. Your comments will be publicly available.

comments powered by Disqus

Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend