Organism : Synechococcus elongatus PCC 7942 | Module List:
Module 80 Profile

GeneModule member RegulatorRegulator MotifMotif
Network Help

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges.

Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif
Regulators for Module 80

There are 0 regulatory influences for Module 80

Warning: No Regulators were found!

Regulator Help

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type.

tf: Transcription factor

ef: Environmental factor

combiner: Combinatorial influence of a tf or an ef through logic gate. Table is sortable by clicking on the arrows next to column headers.

Motif information (de novo identified motifs for modules)

There are 3 motifs predicted.

Motif Table (3)
Motif Id e-value Consensus Motif Logo
1760 1.50e+02 aAgAGCtGa.tGAtt
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1761 9.00e+03 TtTtATtCaAtT
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1762 5.10e+03 TcTGATTGcTG
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment

Regulon 80 is enriched for following functions.

KEGG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini Hochberg pvalue Genes with function Method
Carbohydrate Metabolism kegg subcategory 7.75e-03 3.72e-02 5/24
Membrane Transport kegg subcategory 3.89e-03 2.48e-02 3/24
Carbohydrate Metabolism kegg subcategory 2.09e-02 4.54e-02 3/24
Environmental Information Processing kegg category 1.75e-02 4.00e-02 3/24
Membrane Transport kegg subcategory 3.89e-03 1.65e-02 3/24
ABC transporters kegg pathway 2.13e-03 1.22e-02 3/24

COG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
Defense mechanisms cog subcategory 6.00e-06 7.70e-04 4/24
Signal transduction mechanisms cog subcategory 2.64e-03 4.37e-02 4/24
Cellular processes and signaling cog category 1.75e-03 3.29e-03 9/24
Defense mechanisms cog subcategory 6.00e-06 1.80e-05 4/24
Signal transduction mechanisms cog subcategory 2.64e-03 4.85e-03 4/24
Functions Help

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Members for Module 80

There are 24 genes in Module 80

Gene Member Table (24)
Name Common name Type Gene ID Chromosome Start End Strand Description TF
Synpcc7942_0082 CDS 3773799 chromosome 79265 81013 + ATPase False
Synpcc7942_0127 CDS 3773467 chromosome 126390 127058 + "transcriptional regulator, Crp/Fnr family (IMGterm)" True
Synpcc7942_0937 CDS 3775324 chromosome 943435 944127 - hypothetical protein (EC:3.1.2.6) False
Synpcc7942_1036 CDS 3773966 chromosome 1050754 1051257 + hypothetical protein False
Synpcc7942_1216 CDS 3773504 chromosome 1238550 1240109 - circadian clock protein KaiC (IMGterm) False
Synpcc7942_1217 CDS 3773505 chromosome 1240159 1240467 - circadian clock protein KaiB False
Synpcc7942_1241 CDS 3773529 chromosome 1265088 1266137 + nitrite reductase related protein False
Synpcc7942_1485 CDS 3773657 chromosome 1536407 1536673 - hypothetical protein False
Synpcc7942_1520 rpsT CDS 3774944 chromosome 1572486 1572782 + SSU ribosomal protein S20P (IMGterm) False
Synpcc7942_1521 CDS 3774945 chromosome 1572849 1573646 + Sec-independent protein translocase TatD (EC 3.1.21.-) (IMGterm) False
Synpcc7942_1561 CDS 3774985 chromosome 1618181 1618687 - hypothetical protein False
Synpcc7942_1608 CDS 3775675 chromosome 1673935 1675344 + "mannose-6-phosphate isomerase, type 2 (EC 5.3.1.8) / mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22) (IMGterm)" False
Synpcc7942_1773 CDS 3774348 chromosome 1839008 1839325 + hypothetical protein False
Synpcc7942_1817 CDS 3774392 chromosome 1891423 1891788 - response regulator receiver domain protein (CheY-like) False
Synpcc7942_1848 CDS 3774423 chromosome 1914772 1915836 - aspartate semialdehyde dehydrogenase (EC 1.2.1.11) (IMGterm) False
Synpcc7942_1956 CDS 3774141 chromosome 2028196 2029113 + acetyl-CoA carboxylase carboxyltransferase subunit alpha (IMGterm) False
Synpcc7942_1966 CDS 3774153 chromosome 2037413 2037670 + hypothetical protein False
Synpcc7942_2042 CDS 3774261 chromosome 2111810 2112136 - hypothetical protein False
Synpcc7942_2109 CDS 3774328 chromosome 2189141 2190916 + ATPase False
Synpcc7942_2148 CDS 3773705 chromosome 2230823 2231845 + ATPase False
Synpcc7942_2149 CDS 3773706 chromosome 2231897 2232757 + ABC-2 type transport system permease protein False
Synpcc7942_2174 CDS 3773731 chromosome 2253875 2254504 + "putative transcriptional regulator, Crp/Fnr family" True
Synpcc7942_2456 CDS 3774476 chromosome 2534175 2535125 + hypothetical protein False
Synpcc7942_2496 CDS 3774516 chromosome 2576687 2577886 - "amino acid/amide ABC transporter substrate-binding protein, HAAT family (TC 3.A.1.4.-) (IMGterm)" False

Genes Help

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Help

What is a module?

Regulatory units (modules) in the Network Portal are based on the network inference algorithm used. For the current version, modules are based on cMonkey modules and Inferelator regulatory influences on these modules. More specifically, module refers to set of genes that are conditionally co-regulated under subset of the conditions. Identification of modules integrates co-expression, de-novo motif identification, and other functional associations such as operon information and protein-protein interactions.

Module Overview

The landing module page shows quick summary info including co-expression profiles, de-novo identified motifs, and transcription factors and/or environmental factors as regulatory influences. It also includes module residual, motif e-values, conditions and links to other resources such as NCBI and Microbesonline. . If a transcription factor is included in the manually curated RegPrecise database, further information from RegPrecise is shown, allowing users to perform comparative analysis.

Expression Profiles

Expression profiles is a plot of the expression ratios (log10) of the module's genes, over all subset of the conditions included in the module. The X-axis represent conditions and the Y-axis represents log10 expression ratios. Each gene is plotted as line plot with different colors. Colored legend for the lines are presented under the plot. This plot is dynamic. Clicking on the gene names in the legend will show/hide the plot for that particular gene. A tooltip will show expression ratio information if you mouseover the lines in the plot.

Motif Locations

Location of the Identified motifs for the module in the upstream regions of the member genes are shown under the expression profiles plot. This plot shows the diagram of the upstream positions of the motifs, colored red and green for motifs #1, and 2, respectively. Intensity of the color is proportional to the significance of the occurence of that motif at a given location. Motifs on the forward and reverse strand are represented over and under the line respectively.

Network

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges. Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif

Regulators

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type. tf: Transcription factor, ef: Environmental factor and combiner:Combinatorial influence of a tf or an ef through logic gate. Tabel is sortable by clicking on the arrows next to column headers.

Motifs

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Functions

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Genes

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Social

You can start a conversation about this module or join the existing discussion by adding your comments. In order to be able to add your comments you need to sign in by using any of the following services;Disqus, Google, Facebook or Twitter. For full compatibility with other network portal features, we recommend using your Google ID.

Definitions

Residual: is a measure of bicluster quality. Mean bicluster residual is smaller when the expression profile of the genes in the module is "tighter". So smaller residuals are usually indicative of better bicluster quality.

Expression Profile: is a preview of the expression profiles of all the genes under subset of conditions included in the module. Tighter expression profiles are usually indicative of better bicluster quality.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Genes: Number of genes included in the module.

Functions: We identify functional enrichment of each module by camparing to different functional categories such as KEGG, COG, GO etc. by using hypergeometric function. If the module is significantly enriched for any of the functions, this column will list few of the these functions as an overview. Full list of functions is available upon visiting the module page under the Functions tab.