Organism : Clostridium acetobutylicum | Module List :
CAC3346

MDR-type permease (NCBI ptt file)

CircVis
Functional Annotations (4)
Function System
tetracycline:hydrogen antiporter activity go/ molecular_function
tetracycline transport go/ biological_process
integral to membrane go/ cellular_component
response to antibiotic go/ biological_process
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for CAC3346
(Mouseover regulator name to see its description)

CAC3346 is regulated by 15 influences and regulates 0 modules.
Regulators for CAC3346 (15)
Regulator Module Operator
CAC0093 150 tf
CAC0255 150 tf
CAC1280 150 tf
CAC2236 150 tf
CAC3370 150 tf
CAC3433 150 tf
CAC3488 150 tf
CAC3651 150 tf
CAC0627 4 tf
CAC1770 4 tf
CAC2552 4 tf
CAC3166 4 tf
CAC3236 4 tf
CAC3409 4 tf
CAC3438 4 tf

Warning: CAC3346 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
6662 3.70e-06 tagGAGGa
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6663 4.40e+02 ACAGGaTGG
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6952 3.80e-09 gGAGGtaA
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6953 6.30e+03 GACTATGTTTGAGAAAATTGAGC
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for CAC3346

CAC3346 is enriched for 4 functions in 2 categories.
Enrichment Table (4)
Function System
tetracycline:hydrogen antiporter activity go/ molecular_function
tetracycline transport go/ biological_process
integral to membrane go/ cellular_component
response to antibiotic go/ biological_process
Module neighborhood information for CAC3346

CAC3346 has total of 64 gene neighbors in modules 4, 150
Gene neighbors (64)
Gene Common Name Description Module membership
CAC0075 CAC0075 Ferredoxin (NCBI ptt file) 69, 150
CAC0124 CAC0124 Hypothetical protein (NCBI ptt file) 108, 150
CAC0133 CAC0133 Hypothetical protein, CF-4 family (NCBI ptt file) 150, 300
CAC0206 CAC0206 Uncharacterized conserved membrane protein (NCBI ptt file) 98, 150
CAC0263 serB Phosphoserine phosphatase related protein (NCBI ptt file) 4, 218
CAC0309 CAC0309 Cell wall-associated hydrolase (NCBI ptt file) 4, 86
CAC0312 CAC0312 Hypothetical protein (NCBI ptt file) 4, 172
CAC0313 CAC0313 Phage shock protein A (NCBI ptt file) 4, 172
CAC0538 CAC0538 Beta-mannanase ManB-like enzyme, contains ChW-repeats (NCBI ptt file) 4, 205
CAC0577 CAC0577 Endo-arabinase related enzyme (family 43 glycosyl hydrolase domain and ricin B-like domain) (NCBI ptt file) 150, 216
CAC0697 CAC0697 Galactose mutarotase related enzyme (NCBI ptt file) 4, 170
CAC0719 CAC0719 Epoxide hydrolase, similar to eukaryotic (NCBI ptt file) 150, 356
CAC0722 CAC0722 Predicted membrane protein; CF-9 family (NCBI ptt file) 4, 309
CAC0813 CAC0813 Hypothetical protein (NCBI ptt file) 123, 150
CAC0955 CAC0955 ATP-dependent Zn protease (NCBI ptt file) 150, 190
CAC1048 CAC1048 Carbonic anhydrases/acetyltransferases, isoleucine patch superfamily (NCBI ptt file) 4, 253
CAC1139 CAC1139 Phage related protein, YonO B.subtilis homolog (NCBI ptt file) 4, 16
CAC1165 CAC1165 Hypothetical protein (NCBI ptt file) 4, 104
CAC1184 CAC1184 Hypothetical protein (NCBI ptt file) 4, 16
CAC1215 CAC1215 Hypothetical protein (NCBI ptt file) 4, 176
CAC1319 glpF Glycerol uptake facilitator protein, GLPF (NCBI ptt file) 4, 133
CAC1320 glpP Glycerol-3-phosphate responsive antiterminator (mRNA-binding), GLPP (NCBI ptt file) 4, 86
CAC1332 uxuA D-mannonate hydrolase (NCBI ptt file) 150, 169
CAC1359 CAC1359 Predicted xylanase/chitin deacetylase (NCBI ptt file) 150, 190
CAC1458 CAC1458 PTS system, fructose(mannose)-specific IIB (NCBI ptt file) 136, 150
CAC1464 CAC1464 UVRA-like protein, probably involved in MDR transport (NCBI ptt file) 4, 166
CAC1521 CAC1521 Uncharacterized membrane protein, yetF/ydfS/ykjA/yrbG/ydfR B.subtilis ortholog (NCBI ptt file) 150, 208
CAC1536 CAC1536 Transcriptional regulator, AcrR family (NCBI ptt file) 150, 165
CAC1606 CAC1606 Uncharacterized conserved protein (NCBI ptt file) 4, 73
CAC1607 CAC1607 Hypothetical protein (NCBI ptt file) 4, 137
CAC2050 CAC2050 Predicted membrane protein (NCBI ptt file) 4, 110
CAC2257 CAC2257 Hypothetical protein (NCBI ptt file) 4, 69
CAC2322 CAC2322 Hypothetical protein (NCBI ptt file) 4, 351
CAC2504 CAC2504 Hypothetical protein (NCBI ptt file) 4, 53
CAC2583 CAC2583 Uncharacterized conserved membrane protein, YHGE B.subtilis homolog (NCBI ptt file) 150, 300
CAC2587 CAC2587 GGDEF domain containing protein (NCBI ptt file) 150, 169
CAC2588 CAC2588 Glycosyltransferase (NCBI ptt file) 150, 169
CAC2596 CAC2596 Rieske FeS-domain containing oxidoreductase (NCBI ptt file) 65, 150
CAC2689 CAC2689 Uncharacterized protein, YJCM B.subtilis ortholog (NCBI ptt file) 4, 216
CAC2691 CAC2691 D-lactate dehydrogenase (NCBI ptt file) 150, 338
CAC2761 CAC2761 Thiamine biosynthesis lipoprotein ApbE (NCBI ptt file) 150, 183
CAC2762 CAC2762 Polyferredoxin (NCBI ptt file) 26, 150
CAC2992 CAC2992 Predicted short-chain dehydrogenase (NCBI ptt file) 4, 132
CAC3087 CAC3087 Phosphoenolpyruvate-protein kinase (PTS system enzyme I) (NCBI ptt file) 4, 255
CAC3240 CAC3240 Predicted membrane protein (NCBI ptt file) 13, 150
CAC3241 CAC3241 Uncharacterized conserved membrane protein, YYAD B.subtilis ortholog (NCBI ptt file) 150, 301
CAC3264 CAC3264 Uncharacterized conserved protein, YTFJ B.subtilis ortholog (NCBI ptt file) 4, 341
CAC3280 CAC3280 Possible surface protein, responsible for cell interaction; contains cell adhesion domain and ChW-repeats (NCBI ptt file) 4, 86
CAC3332 CAC3332 Predicted amidohydrolase (dihydroorothase family) (NCBI ptt file) 150, 360
CAC3341 CAC3341 Multimeric flavodoxin WrbA family protein (NCBI ptt file) 4, 341
CAC3346 CAC3346 MDR-type permease (NCBI ptt file) 4, 150
CAC3361 CAC3361 Transcriptional regulator, LysR family (NCBI ptt file) 150, 240
CAC3370 CAC3370 Predicted transcriptional regulator (NCBI ptt file) 150, 240
CAC3430 CAC3430 Membrane associated, signal transduction histidine kinase-like ATPase (NCBI ptt file) 4, 353
CAC3433 CAC3433 Transcriptional regulators, AcrR family (NCBI ptt file) 150, 344
CAC3548 CAC3548 Uncharacterized conserved Zn-finger containing protein (NCBI ptt file) 4, 244
CAC3549 CAC3549 Subtilisin-like serine protease (NCBI ptt file) 150, 190
CAC3597 CAC3597 Rubrerythrin (NCBI ptt file) 4, 57
CAC3598 CAC3598 Rubrerythrin (NCBI ptt file) 4, 57
CAC3599 CAC3599 Hypothetical protein (NCBI ptt file) 4, 206
CAC3609 CAC3609 ABC-type MDR transport system, permease component (NCBI ptt file) 26, 150
CAC3610 CAC3610 ABC-type MDR transport system, permease component (NCBI ptt file) 24, 150
CAC3623 CAC3623 Activator of 2-hydroxyglutaryl-CoA dehydratase (HSP70-class ATPase domain) (NCBI ptt file) 4, 30
CAC3697 CAC3697 Uncharacterized, Zn-finger domain containing protein, YXKC B.subtilis homolog (NCBI ptt file) 123, 150
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for CAC3346
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend