Rv0014c Serine/threonine-protein kinase PknB (EC 2.7.11.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0014c pknB Serine/threonine-protein kinase PknB (EC 2.7.11.1) CDS 15590 17470 - 1 881 626 FALSE

Rv0014c (Serine/threonine-protein kinase PknB (EC 2.7.11.1)) is predicted to be co-regulated in modules bicluster_0270 with residual 0.54 and bicluster_0329 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 890.00 and 32,000.00 for bicluster_0270 and 0.00 and 1.10 for bicluster_0329 respectively.

These modules are enriched for following go terms: inositol metabolic process, inositol biosynthetic process, polyol biosynthetic process, acyl-CoA dehydrogenase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No -27 0.01 0.970235 CDS
Motif 1 Motif 2 Residual
bicluster_0270
e.value: 
890
Motif Bicluster: 
e.value: 
32000
Motif Bicluster: 
0.54
bicluster_0329
e.value: 
0.00000000023
Motif Bicluster: 
e.value: 
1.1
Motif Bicluster: 
0.54
Product (LegacyBRC) Product (RefSeq)
Serine_threonine-protein kinase pknB transmembrane serine/threonine-protein kinase B PKNB (protein kinase B) (STPK B)
Operon # Operon
10 - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607156 NP_214528.1 Run
GO:0004672

protein kinase activity

protein kinase activity

Details: 
Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.
GO Category: 
molecular_function
18
Total items in this category:  
GO:0004674

protein serine/threonine kinase activity

protein serine/threonine kinase activity

Details: 
Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
GO Category: 
molecular_function
10
Total items in this category:  
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0008360

regulation of cell shape

regulation of cell shape

Details: 
Any process that modulates the surface configuration of a cell.
GO Category: 
biological_process
4
Total items in this category:  
GO:0030145

manganese ion binding

manganese ion binding

Details: 
Interacting selectively and non-covalently with manganese (Mn) ions.
GO Category: 
molecular_function
31
Total items in this category:  
GO:0032091

negative regulation of protein binding

negative regulation of protein binding

Details: 
Any process that stops, prevents, or reduces the frequency, rate or extent of protein binding.
GO Category: 
biological_process
2
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0043085

positive regulation of catalytic activity

positive regulation of catalytic activity

Details: 
Any process that activates or increases the activity of an enzyme.
GO Category: 
biological_process
7
Total items in this category:  
GO:0043086

negative regulation of catalytic activity

negative regulation of catalytic activity

Details: 
Any process that stops or reduces the activity of an enzyme.
GO Category: 
biological_process
7
Total items in this category:  
GO:0043388

positive regulation of DNA binding

positive regulation of DNA binding

Details: 
Any process that increases the frequency, rate or extent of DNA binding. DNA binding is any process in which a gene product interacts selectively with DNA (deoxyribonucleic acid).
GO Category: 
biological_process
3
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: