Rv0016c Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0016c pbpA Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129) CDS 18759 20234 - 1 476 491 FALSE

Rv0016c (Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129)) is predicted to be co-regulated in modules bicluster_0329 with residual 0.54 and bicluster_0456 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 1.10 for bicluster_0329 and 190.00 and 9,400.00 for bicluster_0456 respectively.

These modules are enriched for following go terms: inositol metabolic process, inositol biosynthetic process, polyol biosynthetic process, acyl-CoA dehydrogenase activity .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 16:54
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15134 MT0019 1427
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, TetR family
No -124 -0.13 0.762306 Internal.TSS
Transcriptional regulator, AsnC family
No 9 -0.11 0.900955 CDS
Motif 1 Motif 2 Residual
bicluster_0329
e.value: 
0.00000000023
Motif Bicluster: 
e.value: 
1.1
Motif Bicluster: 
0.54
bicluster_0456
e.value: 
190
Motif Bicluster: 
e.value: 
9400
Motif Bicluster: 
0.56
Product (LegacyBRC) Product (RefSeq)
Penicillin-binding protein A penicillin-binding protein PbpA
Operon # Operon
10 - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Peptidoglycan glycosyltransferase Peptidoglycan biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Peptidoglycan biosynthesis

16
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607158 NP_214530.1 Run
GO:0008955

peptidoglycan glycosyltransferase activity

peptidoglycan glycosyltransferase activity

Details: 
Catalysis of the reaction: [GlcNAc-(1,4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-diphosphoundecaprenol + GlcNAc-(1,4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = [GlcNAc-(1,4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-diphosphoundecaprenol + undecaprenyl diphosphate.
GO Category: 
molecular_function
5
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0008658

penicillin binding

penicillin binding

Details: 
Interacting selectively and non-covalently with penicillin, any antibiotic that contains the condensed beta-lactamthiazolidine ring system.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0030428

cell septum

cell septum

Details: 
A structure composed of peptidoglycan and often chitin in addition to other materials. It usually forms perpendicular to the long axis of a cell or hypha and grows centripetally from the cell wall to the center of the cell and often functions in the compartmentalization of a cell into two daughter cells.
GO Category: 
cellular_component
1
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.270000 0.92

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: