Rv0018c Serine/threonine phosphatase PPP (EC 3.1.3.16)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0018c ppp Serine/threonine phosphatase PPP (EC 3.1.3.16) CDS 21637 23181 - 1 545 514 FALSE

Rv0018c (Serine/threonine phosphatase PPP (EC 3.1.3.16)) is predicted to be co-regulated in modules bicluster_0270 with residual 0.54 and bicluster_0329 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 890.00 and 32,000.00 for bicluster_0270 and 0.00 and 1.10 for bicluster_0329 respectively.

These modules are enriched for following go terms: inositol metabolic process, inositol biosynthetic process, polyol biosynthetic process, acyl-CoA dehydrogenase activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Distance to Tuberculist Start Codon Internal TSS New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-2.077 615 22566 198 23370 23172 23181
Last update: 10/16/2017 - 16:54
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14771 MT0021 295
Product (LegacyBRC) Product (RefSeq)
PP2C-family Ser_Thr phosphatase serine/threonine phosphatase
Operon # Operon
10 - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607160 NP_214532.1 Run
GO:0000287

magnesium ion binding

magnesium ion binding

Details: 
Interacting selectively and non-covalently with magnesium (Mg) ions.
GO Category: 
molecular_function
52
Total items in this category:  
GO:0004647

phosphoserine phosphatase activity

phosphoserine phosphatase activity

Details: 
Catalysis of the reaction: L(or D)-O-phosphoserine + H2O = L(or D)-serine + phosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0004721

phosphoprotein phosphatase activity

phosphoprotein phosphatase activity

Details: 
Catalysis of the reaction: a phosphoprotein + H2O = a protein + phosphate. Together with protein kinases, these enzymes control the state of phosphorylation of cell proteins and thereby provide an important mechanism for regulating cellular activity.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0005887

integral to plasma membrane

integral to plasma membrane

Details: 
Penetrating at least one phospholipid bilayer of a plasma membrane. May also refer to the state of being buried in the bilayer with no exposure outside the bilayer.
GO Category: 
cellular_component
101
Total items in this category:  
GO:0006469

negative regulation of protein kinase activity

negative regulation of protein kinase activity

Details: 
Any process that stops, prevents, or reduces the frequency, rate or extent of protein kinase activity.
GO Category: 
biological_process
1
Total items in this category:  
GO:0006470

protein dephosphorylation

protein dephosphorylation

Details: 
The process of removing one or more phosphoric residues from a protein.
GO Category: 
biological_process
3
Total items in this category:  
GO:0016021

integral to membrane

integral to membrane

Details: 
Penetrating at least one phospholipid bilayer of a membrane. May also refer to the state of being buried in the bilayer with no exposure outside the bilayer. When used to describe a protein, indicates that all or part of the peptide sequence is embedded in the membrane.
GO Category: 
cellular_component
6
Total items in this category:  
GO:0030145

manganese ion binding

manganese ion binding

Details: 
Interacting selectively and non-covalently with manganese (Mn) ions.
GO Category: 
molecular_function
31
Total items in this category:  
GO:0043085

positive regulation of catalytic activity

positive regulation of catalytic activity

Details: 
Any process that activates or increases the activity of an enzyme.
GO Category: 
biological_process
7
Total items in this category:  
GO:0043086

negative regulation of catalytic activity

negative regulation of catalytic activity

Details: 
Any process that stops or reduces the activity of an enzyme.
GO Category: 
biological_process
7
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.090000 0.99

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: