Rv0262c Aminoglycoside 2'-N-acetyltransferase AAC (AAC(2')-IC)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0262c aac Aminoglycoside 2'-N-acetyltransferase AAC (AAC(2')-IC) CDS 314309 314854 - 546 181 FALSE

Rv0262c (Aminoglycoside 2'-N-acetyltransferase AAC (AAC(2')-IC)) is predicted to be co-regulated in modules bicluster_0141 with residual 0.53 and bicluster_0589 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.31 and 1,600.00 for bicluster_0141 and 0.72 and 69.00 for bicluster_0589 respectively.

These modules are enriched for following go terms: kinase activity.

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, TetR family
Repressed 51 -1.37 0.0000000225 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0141
e.value: 
0.31
Motif Bicluster: 
e.value: 
1600
Motif Bicluster: 
0.53
bicluster_0589
e.value: 
0.72
Motif Bicluster: 
e.value: 
69
Motif Bicluster: 
0.56
Product (LegacyBRC) Product (RefSeq)
Aminoglycoside 2&apos-N-acetyltransferase aminoglycoside 2'-N-acetyltransferase AAC (AAC(2')-IC)
Operon # Operon
181 Rv0262c - Rv0263c - Rv0264c
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607403 NP_214776.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0034069

aminoglycoside N-acetyltransferase activity

aminoglycoside N-acetyltransferase activity

Details: 
Catalysis of the reaction: acetyl-CoA + aminoglycoside = CoA + N-acetylaminoglycoside.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0047921

aminoglycoside 2'-N-acetyltransferase activity

aminoglycoside 2'-N-acetyltransferase activity

Details: 
Catalysis of the reaction: acetyl-CoA + gentamicin C(1a) = N(2')-acetylgentamicin C(1a) + CoA + H(+). This is acetylation of the 2'-amino group of the 6-deoxy-6-aminoglucose ring.
GO Category: 
molecular_function
1
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.920000 5.59

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.