Rv0264c Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0264c Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54) CDS 315783 316415 - 633 210 FALSE

Rv0264c (Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54)) is predicted to be co-regulated in modules bicluster_0141 with residual 0.53 and bicluster_0589 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.31 and 1,600.00 for bicluster_0141 and 0.72 and 69.00 for bicluster_0589 respectively.

These modules are enriched for following go terms: kinase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.859 316436 316415
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
No -32 0.11 0.936811 CDS
Transcriptional regulator, ArsR family
No -50 -0.07 0.91862 CDS
Motif 1 Motif 2 Residual
bicluster_0141
e.value: 
0.31
Motif Bicluster: 
e.value: 
1600
Motif Bicluster: 
0.53
bicluster_0589
e.value: 
0.72
Motif Bicluster: 
e.value: 
69
Motif Bicluster: 
0.56
Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
181 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Allophanate hydrolase Arginine and proline metabolism, Atrazine degradation
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607405 NP_214778.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.450000 1.29

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: