Rv0973c Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0973c accA2 Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4) CDS 1083747 1085750 - 2 004 667 FALSE

Rv0973c (Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)) is predicted to be co-regulated in modules bicluster_0134 with residual 0.52 and bicluster_0394 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 9.50 for bicluster_0134 and 26.00 and 340.00 for bicluster_0394 respectively.

These modules are enriched for following go terms: oxidoreductase activity, acting on other... cellular protein metabolic process, macromolecular complex, cytoplasmic part.

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.264 1085759 1085750
Product (LegacyBRC) Product (RefSeq)
PROBABLE ACETYL-_PROPIONYL-COENZYME A CARBOXYLASE ALPHA CHAIN [ALPHA SUBUNIT] ACCA2: BIOTIN CARBOXYLASE + BIOTIN CARBOXYL CARRIER PROTEIN [BCCP] acetyl-/propionyl-coenzyme A carboxylase subunit alpha
Operon # Operon
649 - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Methylcrotonoyl-CoA carboxylase Valine, leucine and isoleucine degradation
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

Glyoxylate and dicarboxylate metabolism

31
Total items in this category:  

KEGG

Propanoate metabolism

62
Total items in this category:  

KEGG

Carbon fixation pathways in prokaryotes

42
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608113 NP_215488.1 Run
GO:0004485

methylcrotonoyl-CoA carboxylase activity

methylcrotonoyl-CoA carboxylase activity

Details: 
Catalysis of the reaction: 3-methylbut-2-enoyl-CoA + ATP + bicarbonate = trans-3-methylglutaconyl-CoA + ADP + 2 H(+) + phosphate.
GO Category: 
molecular_function
5
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.100000 7.09

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: