Rv0974c Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0974c accD2 Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4) CDS 1085756 1087345 - 1 590 529 FALSE

Rv0974c (Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4)) is predicted to be co-regulated in modules bicluster_0134 with residual 0.52 and bicluster_0326 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 9.50 for bicluster_0134 and 0.00 and 0.40 for bicluster_0326 respectively.

These modules are enriched for following go terms: oxidoreductase activity, acting on other... .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Re-Annotated Start Tuberculist Annotated Start
-1.811 1087351 1087345
Last update: 10/16/2017 - 11:08
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18248 MT1002 1848
Product (LegacyBRC) Product (RefSeq)
PROBABLE ACETYL-_PROPIONYL-CoA CARBOXYLASE [BETA SUBUNIT] ACCD2 acetyl-/propionyl-CoA carboxylase subunit beta
Operon # Operon
649 - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Methylcrotonoyl-CoA carboxylase Valine, leucine and isoleucine degradation
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

Glyoxylate and dicarboxylate metabolism

31
Total items in this category:  

KEGG

Propanoate metabolism

62
Total items in this category:  

KEGG

Carbon fixation pathways in prokaryotes

42
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608114 NP_215489.1 Run
GO:0004485

methylcrotonoyl-CoA carboxylase activity

methylcrotonoyl-CoA carboxylase activity

Details: 
Catalysis of the reaction: 3-methylbut-2-enoyl-CoA + ATP + bicarbonate = trans-3-methylglutaconyl-CoA + ADP + 2 H(+) + phosphate.
GO Category: 
molecular_function
5
Total items in this category:  
GO:0071768

mycolic acid biosynthetic process

mycolic acid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of mycolic acids, beta-hydroxy fatty acids with a long alpha-alkyl side chain.
GO Category: 
biological_process
15
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.450000 0.42

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: