Rv1032c Sensor protein basS/pmrB (EC 2.7.3.-)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1032c trcS Sensor protein basS/pmrB (EC 2.7.3.-) CDS 1156426 1157955 - 1 530 509 FALSE

Rv1032c (Sensor protein basS/pmrB (EC 2.7.3.-)) is predicted to be co-regulated in modules bicluster_0028 with residual 0.52 and bicluster_0299 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 1.50 for bicluster_0028 and 0.00 and 48.00 for bicluster_0299 respectively.

These modules are enriched for following go terms: signal transduction, signaling, single organism signaling, cell communication, response to stimulus, signal transducer activity, molecular transducer activity, phosphorelay sensor kinase activity, receptor activity, signaling receptor activity .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.226 1157886 1157955
Last update: 10/16/2017 - 11:08
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-13503 MT1061 278
Product (LegacyBRC) Product (RefSeq)
Sensor protein two component sensor histidine kinase TRCS
Operon # Operon
691 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Two-component system

53
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608172 NP_215548.1 Run
GO:0004672

protein kinase activity

protein kinase activity

Details: 
Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.
GO Category: 
molecular_function
18
Total items in this category:  
GO:0005509

calcium ion binding

calcium ion binding

Details: 
Interacting selectively and non-covalently with calcium ions (Ca2+).
GO Category: 
molecular_function
3
Total items in this category:  
GO:0030145

manganese ion binding

manganese ion binding

Details: 
Interacting selectively and non-covalently with manganese (Mn) ions.
GO Category: 
molecular_function
31
Total items in this category:  
GO:0046777

protein autophosphorylation

protein autophosphorylation

Details: 
The phosphorylation by a protein of one or more of its own amino acid residues, or residues on an identical protein.
GO Category: 
biological_process
21
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.230000 1.21

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: