Rv1843c Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1843c guaB1 Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205) CDS 2092259 2093698 - 1 440 479 FALSE

Rv1843c (Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205)) is predicted to be co-regulated in modules bicluster_0158 with residual 0.52 and bicluster_0582 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 20,000.00 and 33,000.00 for bicluster_0158 and 0.07 and 0.22 for bicluster_0582 respectively.

These modules are enriched for following go terms: pyruvate dehydrogenase activity, pyruvate dehydrogenase (acetyl-transferr..., oxidoreductase activity, acting on the a... .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-1.371 455 2094150 2093695 2093698
Last update: 10/16/2017 - 11:42
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18228 MT1891 1794
Product (LegacyBRC) Product (RefSeq)
Uncharacterized oxidoreductase Rv1843c_MT1891 inosine 5-monophosphate dehydrogenase
Operon # Operon
1211 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

IMP dehydrogenase Purine metabolism, Drug metabolism - other enzymes
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608980 NP_216359.1 Run
GO:0003938

IMP dehydrogenase activity

IMP dehydrogenase activity

Details: 
Catalysis of the reaction: inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH + H+.
GO Category: 
molecular_function
4
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.400000 1.27

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: