Rv1846c Transcriptional regulator, BlaI family

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1846c blaI Transcriptional regulator, BlaI family CDS 2096183 2096599 - 417 138 TRUE

Rv1846c (Transcriptional regulator, BlaI family) is predicted to be co-regulated in modules bicluster_0289 with residual 0.53 and bicluster_0502 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.03 for bicluster_0289 and 0.00 and 1.20 for bicluster_0502 respectively.

These modules are enriched for following go terms: oxidoreductase activity, acting on the C..., oxidoreductase activity, acting on CH-OH... .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 10 of 11
Gene Target Differential Expression Distance Expression pvalue Type
No -15 0.17 0.863321 Primary.TSS
Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8)
Repressed -36 -0.75 0.00000000344 Primary.TSS
Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB
Repressed -2 -0.74 0.000000000186 Primary.TSS
Cytochrome oxidase assembly protein
Repressed 32 -1.65 0.0000000000119 Internal.TSS
Cytochrome oxidase assembly protein
Repressed 14 -1.65 0.0000000000119 CDS
Cytochrome d ubiquinol oxidase subunit I (EC 1.10.3.-)
Repressed 16 -1.22 0.0113385 Primary.TSS
Transcriptional regulator, BlaI family
No 45 0.21 0.733447 Primary.TSS
Putative esterase
No -93 0.12 0.911849 Primary.TSS
RNA polymerase sigma-54 factor RpoN
Repressed -44 -1.44 0.00000011 Primary.TSS
Inner membrane protein translocase component YidC, long form
No -139 -0.39 0.467463 Primary.TSS
Displaying 1 - 5 of 5
ChipSeq TF Differential Expression Distance Expression pvalue Type
No -142 0.01 0.988325 Primary.TSS
Transcriptional regulator, TetR family
No -42 0.29 0.299455 Primary.TSS
Transcriptional regulator, MarR family
No -42 -0.56 0.0119001 Primary.TSS
Transcriptional regulatory protein
No 45 0.3 0.12745 Primary.TSS
Transcriptional regulator, BlaI family
No 45 0.21 0.733447 Primary.TSS
Product (LegacyBRC) Product (RefSeq)
POSSIBLE TRANSCRIPTIONAL REGULATORY PROTEIN transcriptional regulatory protein
Operon # Operon
1211 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608983 NP_216362.1 Run
GO:0003677

DNA binding

DNA binding

Details: 
Any molecular function by which a gene product interacts selectively with DNA (deoxyribonucleic acid).
GO Category: 
molecular_function
37
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
GO:0006355

regulation of transcription, DNA-dependent

regulation of transcription, DNA-dependent

Details: 
Any process that modulates the frequency, rate or extent of cellular DNA-dependent transcription.
GO Category: 
biological_process
15
Total items in this category:  
GO:0046677

response to antibiotic

response to antibiotic

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an antibiotic stimulus. An antibiotic is a chemical substance produced by a microorganism which has the capacity to inhibit the growth of or to kill other microorganisms.
GO Category: 
biological_process
15
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
BioProject Accession GEO Series References Repository Sample Method Sample Type
PRJNA254351 GSM1426735 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426736 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426737 GSE59086 25232098 GEO Tiling Array RNA
Experiment UCSC Genome Browser
Rv1846c_B461 UCSC Browser Tracks
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.700000 1.50

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.0000340849
p-value INH: 0.00139529
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 13.59 U
D3I 3 3 3.83 13.34 I
D3U 3 3 3.83 13.04 U
D5I 9 5 6.00 12.69 I
D5U 17 5 6.00 12.89 U
D7I 18 7 8.14 12.45 I
D7U 19 7 8.14 13.46 U
D14I 4 14 15.63 9.37 I
D14U 4 14 15.63 12.52 U
D17I 3 17 19.15 6.48 I
D17U 3 17 19.15 11.89 U
D21I 4 21 23.23 6.25 I
D21U 4 21 23.23 12.06 U
D24I 3 24 26.60 4.28 I
D24U 3 24 26.60 11.25 U
D28I 4 28 30.61 5.72 I
D28U 4 28 30.61 11.17 U