Rv2070c Cobalt-precorrin-6x reductase (EC 1.3.1.54)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2070c cobK Cobalt-precorrin-6x reductase (EC 1.3.1.54) CDS 2327491 2328225 - 735 244 FALSE

Rv2070c (Cobalt-precorrin-6x reductase (EC 1.3.1.54)) is predicted to be co-regulated in modules bicluster_0057 with residual 0.63 and bicluster_0497 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.02 for bicluster_0057 and 310.00 and 800.00 for bicluster_0497 respectively.

These modules are enriched for following go terms: tetrapyrrole metabolic process, tetrapyrrole biosynthetic process, vitamin metabolic process, water-soluble vitamin metabolic process, vitamin biosynthetic process, water-soluble vitamin biosynthetic proce... 3-hydroxyacyl-CoA dehydrogenase activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 12:06
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18626 MT2130 2712
Product (LegacyBRC) Product (RefSeq)
Precorrin-6A reductase cobalt-precorrin-6x reductase
Operon # Operon
1354 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Precorrin-6A reductase Porphyrin and chlorophyll metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Porphyrin and chlorophyll metabolism

31
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609207 NP_216586.1 Run
GO:0016994

precorrin-6A reductase activity

precorrin-6A reductase activity

Details: 
Catalysis of the reaction: precorrin-6B + NADP+ = precorrin-6A + NADPH + H+.
GO Category: 
molecular_function
1
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.040000 0.71

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: