Rv2071c Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2071c cobM Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133) CDS 2328222 2328977 - 756 251 FALSE

Rv2071c (Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133)) is predicted to be co-regulated in modules bicluster_0057 with residual 0.63 and bicluster_0497 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.02 for bicluster_0057 and 310.00 and 800.00 for bicluster_0497 respectively.

These modules are enriched for following go terms: tetrapyrrole metabolic process, tetrapyrrole biosynthetic process, vitamin metabolic process, water-soluble vitamin metabolic process, vitamin biosynthetic process, water-soluble vitamin biosynthetic proce... 3-hydroxyacyl-CoA dehydrogenase activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 12:06
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15073 MT2131 1313
Product (LegacyBRC) Product (RefSeq)
Precorrin-4 C[11]-methyltransferase precorrin-4 C11-methyltransferase CobM
Operon # Operon
1354 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Precorrin-4 C(11)-methyltransferase Porphyrin and chlorophyll metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Porphyrin and chlorophyll metabolism

31
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609208 NP_216587.1 Run
GO:0046026

precorrin-4 C11-methyltransferase activity

precorrin-4 C11-methyltransferase activity

Details: 
Catalysis of the reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin 5.
GO Category: 
molecular_function
1
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.330000 0.51

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: