Rv2383c Phenyloxazoline synthase MbtB (EC 6.3.2.-)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2383c mbtB Phenyloxazoline synthase MbtB (EC 6.3.2.-) CDS 2671593 2675837 - 4 245 1 414 FALSE

Rv2383c (Phenyloxazoline synthase MbtB (EC 6.3.2.-)) is predicted to be co-regulated in modules bicluster_0502 with residual 0.52 and bicluster_0525 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 1.20 for bicluster_0502 and 0.00 and 5.20 for bicluster_0525 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Phenyloxazoline synthase mbtB phenyloxazoline synthase MBTB (phenyloxazoline synthetase)
Operon # Operon
1571 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Biosynthesis of siderophore group nonribosomal peptides

9
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609520 NP_216899.1 Run
GO:0000036

ACP phosphopantetheine attachment site binding involved in fatty acid biosynthetic process

ACP phosphopantetheine attachment site binding involved in fatty acid biosynthetic process

Details: 
Interacting selectively with the attachment site of the phosphopantetheine prosthetic group of an acyl carrier protein (ACP) as part of the process of fatty acid biosynthesis.
GO Category: 
molecular_function
5
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0009405

pathogenesis

pathogenesis

Details: 
The set of specific processes that generate the ability of an organism to cause disease in another.
GO Category: 
biological_process
65
Total items in this category:  
GO:0010106

cellular response to iron ion starvation

cellular response to iron ion starvation

Details: 
Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of iron ions.
GO Category: 
biological_process
14
Total items in this category:  
GO:0019290

siderophore biosynthetic process

siderophore biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of siderophores, low molecular weight Fe(III)-chelating substances made by aerobic or facultatively anaerobic bacteria, especially when growing under iron deficient conditions. The complexes of Fe(3+)-siderophores have very high stability constants and are taken up by specific transport systems by microorganisms; the subsequent release of iron requires enzymatic action.
GO Category: 
biological_process
6
Total items in this category:  
GO:0031177

phosphopantetheine binding

phosphopantetheine binding

Details: 
Interacting selectively with phosphopantetheine, the vitamin pantetheine 4'-(dihydrogen phosphate).
GO Category: 
molecular_function
5
Total items in this category:  
GO:0052572

response to host immune response

response to host immune response

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of detecting the immune response of the host organism. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
62
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.020000 1.00

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: