Rv2858c Aldehyde dehydrogenase in hypothetical Actinobacterial gene cluster

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2858c aldC Aldehyde dehydrogenase in hypothetical Actinobacterial gene cluster CDS 3169356 3170723 - 1 368 455 FALSE

Rv2858c (Aldehyde dehydrogenase in hypothetical Actinobacterial gene cluster) is predicted to be co-regulated in modules bicluster_0083 with residual 0.52 and bicluster_0177 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 240.00 and 440.00 for bicluster_0083 and 3.10 and 110.00 for bicluster_0177 respectively.

These modules are enriched for following go terms: glutamine metabolic process glutamine metabolic process.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE ALDEHYDE DEHYDROGENASE ALDC aldehyde dehydrogenase
Operon # Operon
1869 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glycolysis / Gluconeogenesis

35
Total items in this category:  

KEGG

Pentose and glucuronate interconversions

8
Total items in this category:  

KEGG

Ascorbate and aldarate metabolism

5
Total items in this category:  

KEGG

Fatty acid metabolism

51
Total items in this category:  

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

Lysine degradation

46
Total items in this category:  

KEGG

Arginine and proline metabolism

38
Total items in this category:  

KEGG

Histidine metabolism

40
Total items in this category:  

KEGG

Tryptophan metabolism

47
Total items in this category:  

KEGG

beta-Alanine metabolism

37
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609995 NP_217374.1 Run
GO:0004029

aldehyde dehydrogenase (NAD) activity

aldehyde dehydrogenase (NAD) activity

Details: 
Catalysis of the reaction: an aldehyde + NAD+ + H2O = an acid + NADH + H+.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.190000 0.06

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: