Rv2932 Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2932 ppsB Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39) CDS 3251072 3255688 + 4 617 1 538 FALSE

Rv2932 (Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39)) is predicted to be co-regulated in modules bicluster_0247 with residual 0.41 and bicluster_0513 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 430.00 and 9,100.00 for bicluster_0247 and 130.00 and 270.00 for bicluster_0513 respectively.

These modules are enriched for following go terms: glutamate metabolic process, dicarboxylic acid biosynthetic process, intracellular protein transport, protein secretion, secretion by cell, secretion, intracellular transport, cellular protein localization, cellular macromolecule localization, protein transmembrane transporter activi..., P-P-bond-hydrolysis-driven protein trans..., macromolecule transmembrane transporter ..., protein transporter activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Distance to Tuberculist Start Codon Internal TSS New Internal UTR Re-Annotated Start Tuberculist Annotated Start
-0.714 80 3251152 -47 3251105 3251072
Last update: 10/16/2017 - 14:41
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15148 MT3002 1983
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0247
e.value: 
430
Motif Bicluster: 
e.value: 
9100
Motif Bicluster: 
0.41
bicluster_0513
e.value: 
130
Motif Bicluster: 
e.value: 
270
Motif Bicluster: 
0.54
Product (LegacyBRC) Product (RefSeq)
Phenolpthiocerol synthesis polyketide synthase ppsB phenolpthiocerol synthesis type-I polyketide synthase PPSB
Operon # Operon
1915 - - - - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

[Acyl-carrier-protein] S-malonyltransferase Fatty acid biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610069 NP_217448.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.000000 0.94

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: