Rv2930 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2930 fadD26 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) CDS 3243697 3245448 + 1 752 583 FALSE

Rv2930 (Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)) is predicted to be co-regulated in modules bicluster_0276 with residual 0.47 and bicluster_0513 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 93.00 and 170.00 for bicluster_0276 and 130.00 and 270.00 for bicluster_0513 respectively.

These modules are enriched for following go terms: drug transmembrane transporter activity, drug transporter activity glutamate metabolic process, dicarboxylic acid biosynthetic process, intracellular protein transport, protein secretion, secretion by cell, secretion, intracellular transport, cellular protein localization, cellular macromolecule localization, protein transmembrane transporter activi..., P-P-bond-hydrolysis-driven protein trans..., macromolecule transmembrane transporter ..., protein transporter activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:41
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18114 MT2999 1432
Product (LegacyBRC) Product (RefSeq)
Putative fatty-acid--CoA ligase fadD26 acyl-CoA synthetase
Operon # Operon
1915 - - - - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Long-chain-fatty-acid--CoA ligase Fatty acid metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117038 NP_217446.2 Run
GO:0004467

long-chain fatty acid-CoA ligase activity

long-chain fatty acid-CoA ligase activity

Details: 
Catalysis of the reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA; a long-chain fatty acid is a fatty acid with a chain length between C13 and C22.
GO Category: 
molecular_function
13
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0044119

growth of symbiont in host cell

growth of symbiont in host cell

Details: 
The increase in size or mass of symbiont, occurring in its host's cell. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
50
Total items in this category:  
GO:0052572

response to host immune response

response to host immune response

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of detecting the immune response of the host organism. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
62
Total items in this category:  
GO:0070566

adenylyltransferase activity

adenylyltransferase activity

Details: 
Catalysis of the transfer of an adenylyl group to an acceptor.
GO Category: 
molecular_function
6
Total items in this category:  
GO:0071770

DIM/DIP cell wall layer assembly

DIM/DIP cell wall layer assembly

Details: 
The aggregation, arrangement and bonding together of a set of components, including (phenyl)phthiocerol, phthiodiolone, phthiotriol dimycocerosate and diphthioceranate, to form the DIM/DIP layer of the Actinobacterium-type cell wall.
GO Category: 
biological_process
6
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.810000 1.24

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: