Rv2939 Putative conserved polyketide synthase-associated protein PapA5

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2939 papA5 Putative conserved polyketide synthase-associated protein PapA5 CDS 3274949 3276217 + 1 269 422 FALSE

Rv2939 (Putative conserved polyketide synthase-associated protein PapA5) is predicted to be co-regulated in modules bicluster_0276 with residual 0.47 and bicluster_0513 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 93.00 and 170.00 for bicluster_0276 and 130.00 and 270.00 for bicluster_0513 respectively.

These modules are enriched for following go terms: drug transmembrane transporter activity, drug transporter activity glutamate metabolic process, dicarboxylic acid biosynthetic process, intracellular protein transport, protein secretion, secretion by cell, secretion, intracellular transport, cellular protein localization, cellular macromolecule localization, protein transmembrane transporter activi..., P-P-bond-hydrolysis-driven protein trans..., macromolecule transmembrane transporter ..., protein transporter activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:41
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14940 MT3009 327
Product (LegacyBRC) Product (RefSeq)
Phthiocerol_phthiodiolone dimycocerosyl transferase acyltransferase PapA5
Operon # Operon
1916
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610076 NP_217455.1 Run
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0008415

NA

NA

Details: 
NA
GO Category: 
NA
6
Total items in this category:  
GO:0008610

lipid biosynthetic process

lipid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of lipids, compounds soluble in an organic solvent but not, or sparingly, in an aqueous solvent.
GO Category: 
biological_process
13
Total items in this category:  
GO:0071770

DIM/DIP cell wall layer assembly

DIM/DIP cell wall layer assembly

Details: 
The aggregation, arrangement and bonding together of a set of components, including (phenyl)phthiocerol, phthiodiolone, phthiotriol dimycocerosate and diphthioceranate, to form the DIM/DIP layer of the Actinobacterium-type cell wall.
GO Category: 
biological_process
6
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.500000 1.42

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: