Rv2947c (MTCY24G1.02), len: 496 aa, Probable polyketide synthase, almost identical to G560508 PKS002B (495 aa), fasta scores, opt: 3270, E(): 0, (99.6% identity in 496 aa overlap); contains PS00606 Beta-ketoacyl synthases active site; similar to MTCY338.20 (49.9% identity in 465 aa overlap) and MTCY24G1.09 (50.2% identity in 454 aa overlap) and MTCY22H8.03 (47.6% identity in 437 aa overlap)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2947c pks15 (MTCY24G1.02), len: 496 aa, Probable polyketide synthase, almost identical to G560508 PKS002B (495... CDS 3296350 3297840 - 1 491 496 FALSE

Rv2947c ((MTCY24G1.02), len: 496 aa, Probable polyketide synthase, almost identical to G560508 PKS002B (495 aa), fasta scores, opt: 3270, E(): 0, (99.6% identity in 496 aa overlap); contains PS00606 Beta-ketoacyl synthases active site; similar to MTCY338.20 (49.9% identity in 465 aa overlap) and MTCY24G1.09 (50.2% identity in 454 aa overlap) and MTCY22H8.03 (47.6% identity in 437 aa overlap)) is predicted to be co-regulated in modules bicluster_0211 with residual 0.50 and bicluster_0490 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.05 for bicluster_0211 and 0.01 and 730.00 for bicluster_0490 respectively.

These modules are enriched for following go terms: translation, non-membrane-bounded organelle, intracellular non-membrane-bounded organ..., organelle, intracellular organelle, cytoplasmic part .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.865 3297819 3297840
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 4 of 4
ChipSeq TF Differential Expression Distance Expression pvalue Type
Two component DNA binding Transcriptional regulatory protein TcrA
No -72 0.23 0.699673 CDS
No -59 -0.02 0.961584 CDS
putative two-component system response regulator
No -18 0.12 0.807947 CDS
HTH-type transcriptional regulator
No -25 -0.05 0.904253 CDS
Motif 1 Motif 2 Residual
bicluster_0211
e.value: 
0.00000000024
Motif Bicluster: 
e.value: 
0.047
Motif Bicluster: 
0.50
bicluster_0490
e.value: 
0.0085
Motif Bicluster: 
e.value: 
730
Motif Bicluster: 
0.57
Product (LegacyBRC) Product (RefSeq)
PROBABLE POLYKETIDE SYNTHASE PKS15 polyketide synthase PKS15
Operon # Operon
1921 - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610084 NP_217463.1 Run
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0009247

glycolipid biosynthetic process

glycolipid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of glycolipid, a class of 1,2-di-O-acylglycerols joined at oxygen 3 by a glycosidic linkage to a carbohydrate part (usually a mono-, di- or tri-saccharide).
GO Category: 
biological_process
17
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.180000 1.18

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: