Rv2949c Chorismate--pyruvate lyase (EC 4.1.3.40)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv2949c Chorismate--pyruvate lyase (EC 4.1.3.40) CDS 3299971 3300570 - 600 199 FALSE

Rv2949c (Chorismate--pyruvate lyase (EC 4.1.3.40)) is predicted to be co-regulated in modules bicluster_0396 with residual 0.50 and bicluster_0490 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 9.30 for bicluster_0396 and 0.01 and 730.00 for bicluster_0490 respectively.

These modules are enriched for following go terms: organic hydroxy compound transport, tetracycline transport, antibiotic transport, toxin transport, drug transport, response to drug, tetracycline transporter activity, drug:hydrogen antiporter activity, tetracycline:hydrogen antiporter activit..., toxin transporter activity, antibiotic transporter activity, organic hydroxy compound transmembrane t..., solute:cation antiporter activity, solute:hydrogen antiporter activity, antiporter activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Chorismate--pyruvate lyase
Operon # Operon
1921 - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Chorismate lyase Ubiquinone and other terpenoid-quinone biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610086 NP_217465.1 Run
GO:0008813

chorismate lyase activity

chorismate lyase activity

Details: 
Catalysis of the reaction: chorismate = 4-hydroxybenzoate + pyruvate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0046417

chorismate metabolic process

chorismate metabolic process

Details: 
The chemical reactions and pathways involving chorismate, the anion of (3R-trans)-3-((1-carboxyethenyl)oxy)-4-hydroxy-1,5-cyclohexadiene-1-carboxylic acid.
GO Category: 
biological_process
1
Total items in this category:  
GO:0071766

Actinobacterium-type cell wall biogenesis

Actinobacterium-type cell wall biogenesis

Details: 
A cellular process that results in the biosynthesis of constituent macromolecules, assembly, and arrangement of constituent parts of a cell wall of the type found in Actinobacteria. The cell wall is the rigid or semi-rigid envelope lying outside the cell membrane. Actinobacterial cell walls contain characteristic mycolic acids, of which some are covalently linked to the cell wall peptidoglycan and others accumulate at the cell surface.
GO Category: 
biological_process
19
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.070000 7.72

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: