Rv2950c Long-chain fatty-acid-AMP ligase, Mycobacterial subgroup FadD29

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2950c fadD29 Long-chain fatty-acid-AMP ligase, Mycobacterial subgroup FadD29 CDS 3300596 3302344 - 1 749 582 FALSE

Rv2950c (Long-chain fatty-acid-AMP ligase, Mycobacterial subgroup FadD29) is predicted to be co-regulated in modules bicluster_0513 with residual 0.54 and bicluster_0583 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 130.00 and 270.00 for bicluster_0513 and 0.00 and 2.80 for bicluster_0583 respectively.

These modules are enriched for following go terms: glutamate metabolic process, dicarboxylic acid biosynthetic process, intracellular protein transport, protein secretion, secretion by cell, secretion, intracellular transport, cellular protein localization, cellular macromolecule localization, protein transmembrane transporter activi..., P-P-bond-hydrolysis-driven protein trans..., macromolecule transmembrane transporter ..., protein transporter activity O-acyltransferase activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Distance to Tuberculist Start Codon Internal TSS New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.702 1179 3301276 9 3302575 3302566 3302455
Last update: 10/16/2017 - 14:41
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14783 MT3023 147
Product (LegacyBRC) Product (RefSeq)
PROBABLE FATTY-ACID-CoA LIGASE FADD29 [FATTY-ACID-CoA SYNTHETASE] [FATTY-ACID-CoA SYNTHASE] acyl-CoA synthetase
Operon # Operon
1921 - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
161352463 NP_217466.2 Run
GO:0004467

long-chain fatty acid-CoA ligase activity

long-chain fatty acid-CoA ligase activity

Details: 
Catalysis of the reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA; a long-chain fatty acid is a fatty acid with a chain length between C13 and C22.
GO Category: 
molecular_function
13
Total items in this category:  
GO:0070566

adenylyltransferase activity

adenylyltransferase activity

Details: 
Catalysis of the transfer of an adenylyl group to an acceptor.
GO Category: 
molecular_function
6
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.170000 1.17

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: