Rv3264c D-glycero-D-manno-heptose 1-phosphate guanosyltransferase

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3264c manB D-glycero-D-manno-heptose 1-phosphate guanosyltransferase CDS 3644898 3645977 - 1 080 359 FALSE

Rv3264c (D-glycero-D-manno-heptose 1-phosphate guanosyltransferase) is predicted to be co-regulated in modules bicluster_0083 with residual 0.52 and bicluster_0455 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 240.00 and 440.00 for bicluster_0083 and 0.00 and 0.00 for bicluster_0455 respectively.

These modules are enriched for following go terms: glutamine metabolic process .

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0083
e.value: 
240
Motif Bicluster: 
e.value: 
440
Motif Bicluster: 
0.52
bicluster_0455
e.value: 
0.00016
Motif Bicluster: 
e.value: 
0.00028
Motif Bicluster: 
0.53
Product (LegacyBRC) Product (RefSeq)
D-ALPHA-D-MANNOSE-1-PHOSPHATE GUANYLYLTRANSFERASE MANB [D-ALPHA-D-HEPTOSE-1-PHOSPHATE GUANYLYLTRANSFERASE] D-alpha-D-mannose-1-phosphate guanylyltransferase MANB (D-alpha-D-heptose-1-phosphate guanylyltransferase)
Operon # Operon
2137 Rv3264c - Rv3265c - Rv3266c
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fructose and mannose metabolism

18
Total items in this category:  

KEGG

Amino sugar and nucleotide sugar metabolism

28
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117081 YP_177951.1 Run
GO:0009058

biosynthetic process

biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of substances; typically the energy-requiring part of metabolism in which simpler substances are transformed into more complex ones.
GO Category: 
biological_process
4
Total items in this category:  
GO:0016779

nucleotidyltransferase activity

nucleotidyltransferase activity

Details: 
Catalysis of the transfer of a nucleotidyl group to a reactant.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.