Rv3266c dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3266c rmlD dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133) CDS 3646895 3647809 - 915 304 FALSE

Rv3266c (dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133)) is predicted to be co-regulated in modules bicluster_0083 with residual 0.52 and bicluster_0455 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 240.00 and 440.00 for bicluster_0083 and 0.00 and 0.00 for bicluster_0455 respectively.

These modules are enriched for following go terms: glutamine metabolic process .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0083
e.value: 
240
Motif Bicluster: 
e.value: 
440
Motif Bicluster: 
0.52
bicluster_0455
e.value: 
0.00016
Motif Bicluster: 
e.value: 
0.00028
Motif Bicluster: 
0.53
Product (LegacyBRC) Product (RefSeq)
dTDP-6-DEOXY-L-LYXO-4-HEXULOSE REDUCTASE RMLD [DTDP-RHAMNOSE MODIFICATION PROTEIN] [DTDP-RHAMNOSE BIOSYNTHESIS PROTEIN] [DTDP-RHAMNOSE SYNTHASE] dTDP-6-deoxy-L-lyxo-4-hexulose reductase RmlD
Operon # Operon
2137 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Streptomycin biosynthesis

12
Total items in this category:  

KEGG

Polyketide sugar unit biosynthesis

6
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610402 NP_217783.1 Run
GO:0008831

dTDP-4-dehydrorhamnose reductase activity

dTDP-4-dehydrorhamnose reductase activity

Details: 
Catalysis of the reaction: dTDP-6-deoxy-L-mannose + NADP(+) = dTDP-4-dehydro-6-deoxy-L-mannose + H(+) + NADPH.
GO Category: 
molecular_function
1
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: