Rv3266c dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3266c rmlD dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133) CDS 3646895 3647809 - 915 304 FALSE

Rv3266c (dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133)) is predicted to be co-regulated in modules bicluster_0083 with residual 0.52 and bicluster_0455 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 240.00 and 440.00 for bicluster_0083 and 0.00 and 0.00 for bicluster_0455 respectively.

These modules are enriched for following go terms: glutamine metabolic process .

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0083
e.value: 
240
Motif Bicluster: 
e.value: 
440
Motif Bicluster: 
0.52
bicluster_0455
e.value: 
0.00016
Motif Bicluster: 
e.value: 
0.00028
Motif Bicluster: 
0.53
Product (LegacyBRC) Product (RefSeq)
dTDP-6-DEOXY-L-LYXO-4-HEXULOSE REDUCTASE RMLD [DTDP-RHAMNOSE MODIFICATION PROTEIN] [DTDP-RHAMNOSE BIOSYNTHESIS PROTEIN] [DTDP-RHAMNOSE SYNTHASE] dTDP-6-deoxy-L-lyxo-4-hexulose reductase RmlD
Operon # Operon
2137 Rv3264c - Rv3265c - Rv3266c
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Streptomycin biosynthesis

12
Total items in this category:  

KEGG

Polyketide sugar unit biosynthesis

6
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610402 NP_217783.1 Run
GO:0008831

dTDP-4-dehydrorhamnose reductase activity

dTDP-4-dehydrorhamnose reductase activity

Details: 
Catalysis of the reaction: dTDP-6-deoxy-L-mannose + NADP(+) = dTDP-4-dehydro-6-deoxy-L-mannose + H(+) + NADPH.
GO Category: 
molecular_function
1
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.