Rv3536c 2-keto-4-pentenoate hydratase (EC 4.2.1.-)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3536c hsaE 2-keto-4-pentenoate hydratase (EC 4.2.1.-) CDS 3974511 3975296 - 786 261 FALSE

Rv3536c (2-keto-4-pentenoate hydratase (EC 4.2.1.-)) is predicted to be co-regulated in modules bicluster_0199 with residual 0.54 and bicluster_0337 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0199 and 0.00 and 0.00 for bicluster_0337 respectively.

These modules are enriched for following go terms: aspartate carbamoyltransferase activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 15:33
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18845 MT3640 3153
Product (LegacyBRC) Product (RefSeq)
PROBABLE HYDRATASE hydratase
Operon # Operon
2309 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Phenylalanine metabolism

13
Total items in this category:  

KEGG

Benzoate degradation

48
Total items in this category:  

KEGG

Dioxin degradation

5
Total items in this category:  

KEGG

Xylene degradation

4
Total items in this category:  

KEGG

Ethylbenzene degradation

11
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610672 NP_218053.1 Run
GO:0003824

catalytic activity

catalytic activity

Details: 
Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO Category: 
molecular_function
12
Total items in this category:  
GO:0008152

metabolic process

metabolic process

Details: 
The chemical reactions and pathways, including anabolism and catabolism, by which living organisms transform chemical substances. Metabolic processes typically transform small molecules, but also include macromolecular processes such as DNA repair and replication, and protein synthesis and degradation.
GO Category: 
biological_process
2
Total items in this category:  
GO:0016829

lyase activity

lyase activity

Details: 
Catalysis of the cleavage of C-C, C-O, C-N and other bonds by other means than by hydrolysis or oxidation, or conversely adding a group to a double bond. They differ from other enzymes in that two substrates are involved in one reaction direction, but only one in the other direction. When acting on the single substrate, a molecule is eliminated and this generates either a new double bond or a new ring.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0006725

cellular aromatic compound metabolic process

cellular aromatic compound metabolic process

Details: 
The chemical reactions and pathways involving aromatic compounds, any organic compound characterized by one or more planar rings, each of which contains conjugated double bonds and delocalized pi electrons, as carried out by individual cells.
GO Category: 
biological_process
2
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.000000 0.20

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: