Rv3568c 2,3-dihydroxybiphenyl 1,2-dioxygenase

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3568c bphC 2,3-dihydroxybiphenyl 1,2-dioxygenase CDS 4009297 4010199 - 903 300 FALSE

Rv3568c (2,3-dihydroxybiphenyl 1,2-dioxygenase) is predicted to be co-regulated in modules bicluster_0199 with residual 0.54 and bicluster_0200 with residual 0.45.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0199 and 0.00 and 0.16 for bicluster_0200 respectively.

These modules are enriched for following go terms: coenzyme binding, oxidoreductase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE BIPHENYL-23-DIOL 12-DIOXYGENASE BPHC [23OHBP OXYGENASE] [23-DIHYDROXYBIPHENYL DIOXYGENASE] [23-DIHYDROXYBIPHENYL 12-DIOXYGENASE] [DHBD] biphenyl-2,3-diol 1,2-dioxygenase
Operon # Operon
2325 - - - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Chlorocyclohexane and chlorobenzene degradation

7
Total items in this category:  

KEGG

Dioxin degradation

5
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610704 NP_218085.1 Run
GO:0016702

oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen

oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen

Details: 
Catalysis of an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from one donor, and two oxygen atoms is incorporated into a donor.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0070723

response to cholesterol

response to cholesterol

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cholesterol stimulus.
GO Category: 
biological_process
2
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.000000 0.01

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: