Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_2022

Cytochrome b/diheme cytochrome c hybrid protein (NCBI)

CircVis
Functional Annotations (3)
Function System
Cytochrome b cog/ cog
electron transport go/ biological_process
integral to membrane go/ cellular_component
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_2022
(Mouseover regulator name to see its description)

RSP_2022 is regulated by 21 influences and regulates 0 modules.
Regulators for RSP_2022 (21)
Regulator Module Operator
RSP_1225 229 tf
RSP_1231 229 tf
RSP_1435 229 tf
RSP_1952 229 tf
RSP_2494 229 tf
RSP_2533 229 tf
RSP_2681 229 tf
RSP_2889 229 tf
RSP_3621 229 tf
RSP_0607 103 tf
RSP_1225 103 tf
RSP_1231 103 tf
RSP_1607 103 tf
RSP_1776 103 tf
RSP_2026 103 tf
RSP_2171 103 tf
RSP_2494 103 tf
RSP_2867 103 tf
RSP_2922 103 tf
RSP_3179 103 tf
RSP_3616 103 tf

Warning: RSP_2022 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7926 6.50e-02 CcAGgatGAAag
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7927 1.50e+04 AAATAT
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8176 2.00e-05 AAacaGAAGgTCaAaaGagat
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8177 1.40e-06 agTTGcGActCcgAcGAaACTG
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_2022

RSP_2022 is enriched for 3 functions in 3 categories.
Enrichment Table (3)
Function System
Cytochrome b cog/ cog
electron transport go/ biological_process
integral to membrane go/ cellular_component
Module neighborhood information for RSP_2022

RSP_2022 has total of 55 gene neighbors in modules 103, 229
Gene neighbors (55)
Gene Common Name Description Module membership
RSP_0116 RSP_0116 hypothetical protein (NCBI) 14, 229
RSP_0118 RSP_0118 Cytochrome c oxidase, subunit IIc (NCBI) 77, 229
RSP_0134 RSP_0134 hypothetical protein (NCBI) 103, 183
RSP_0209 RSP_0209 possible prophage Lp3 protein 18 (NCBI) 103, 138
RSP_0297 RSP_0297 ABC branched chain amino acid transporter, ATPase subunit (NCBI) 103, 304
RSP_0298 RSP_0298 ABC branched chain amino acid transporter, ATPase subunit (NCBI) 103, 313
RSP_0299 RSP_0299 ABC branched chain amino acid transporter, inner membrane subunit (NCBI) 103, 313
RSP_0300 RSP_0300 ABC branched chain amino acid transporter, inner membrane subunit (NCBI) 103, 147
RSP_0477 RSP_0477 hypothetical protein (NCBI) 97, 103
RSP_0607 RSP_0607 sigma 24 (NCBI) 77, 103
RSP_0617 RSP_0617 hypothetical protein (NCBI) 103, 325
RSP_1294 RSP_1294 possible 2-oxoisovalerate dehydrogenase; E1 component, alpha and beta subunit (NCBI) 103, 217
RSP_1370 RSP_1370 Alpha amylase, catalytic subdomain (NCBI) 124, 229
RSP_1471 RSP_1471 hypothetical protein (NCBI) 140, 229
RSP_1796 sodC Putative Copper/Zinc superoxide dismutase (NCBI) 150, 229
RSP_1822 RSP_1822 hypothetical protein (NCBI) 229, 238
RSP_1933 RSP_1933 Outer membrane protein, OmpA/MotB family (NCBI) 103, 280
RSP_1951 RSP_1951 hypothetical protein (NCBI) 56, 229
RSP_1952 RSP_1952 Cold-shock DNA-binding domain protein (NCBI) 56, 229
RSP_2019 RSP_2019 hypothetical protein (NCBI) 103, 120
RSP_2021 shp Mono-heme class I cytochrome c (NCBI) 150, 229
RSP_2022 RSP_2022 Cytochrome b/diheme cytochrome c hybrid protein (NCBI) 103, 229
RSP_2023 RSP_2023 hypothetical protein (NCBI) 229, 304
RSP_2024 cspA Cold shock protein cspA (NCBI) 56, 229
RSP_2026 RSP_2026 transcriptional regulator, AraC family (NCBI) 103, 176
RSP_2055 RSP_2055 hypothetical protein (NCBI) 103, 379
RSP_2105 RSP_2105 hypothetical protein (NCBI) 103, 325
RSP_2219 RSP_2219 putative lipid A biosynthesis lauroyl acyltransferase (NCBI) 183, 229
RSP_2385 RSP_2385 hypothetical protein (NCBI) 103, 124
RSP_2751 RSP_2751 hypothetical protein (NCBI) 105, 229
RSP_2792 RSP_2792 putative monooxygenase alpha subunit (NCBI) 77, 103
RSP_2793 RSP_2793 Putative reductase component of monooxygenase (NCBI) 103, 313
RSP_2794 RSP_2794 Putative monooxygenase beta subunit (NCBI) 103, 193
RSP_2795 RSP_2795 Putative regulatory protein of multicomponent monooxygenase (NCBI) 103, 193
RSP_2796 RSP_2796 hypothetical protein (NCBI) 77, 103
RSP_2798 groEL3 Putative chaperonin groEL (NCBI) 77, 103
RSP_2807 RSP_2807 putative cytochrome b (NCBI) 14, 229
RSP_2808 RSP_2808 hypothetical protein (NCBI) 14, 229
RSP_2871 aglG ABC alpha-glucoside transporter, inner membrane subunit AglG (NCBI) 229, 235
RSP_2874 RSP_2874 Putative Beta-glucosidase A (NCBI) 229, 233
RSP_2889 RSP_2889 Transcriptional regulator (NCBI) 185, 229
RSP_2890 RSP_2890 Copper-translocating P-type ATPase (NCBI) 185, 229
RSP_2891 RSP_2891 Putative copper chaperone (NCBI) 185, 229
RSP_2964 RSP_2964 hypothetical protein (NCBI) 229, 244
RSP_3230 RSP_3230 hypothetical protein (NCBI) 103, 279
RSP_3334 RSP_3334 HyuE hydantoin racemase (NCBI) 44, 103
RSP_3360 RSP_3360 Adenine specific DNA methyltransferase, D12 class (NCBI) 103, 342
RSP_3497 RSP_3497 Caspase-1, p20 (NCBI) 103, 168
RSP_3498 RSP_3498 Antifreeze protein, type I (NCBI) 103, 370
RSP_3541 RSP_3541 hypothetical protein (NCBI) 103, 242
RSP_3543 RSP_3543 Probable type I restriction-modification system restriction subunit (NCBI) 191, 229
RSP_3576 RSP_3576 hypothetical protein (NCBI) 103, 249
RSP_3621 RSP_3621 Cold-shock DNA-binding protein (NCBI) 56, 229
RSP_3622 RSP_3622 hypothetical protein (NCBI) 56, 229
RSP_3812 RSP_3812 recombinase (NCBI) 103, 189
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_2022
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend